Sequences from confirmation amplicon


Confirmation sequences for line 590A04 and hit At2g22400 are shown.
Line ID 590A04
Locus At2g22400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9506912
Primers used for PCR 22dr - 8474, show primer details
   
Sequence Name 26-K110307-0022-590-A04C-22dr
GenBank Accession LN497976 [GenBank]
Sequencing primer gene specific primer, 22dr: 5'-TAATCATGCAGGTACCTCCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K110307-0022-590-A04C-22dr
TTGACGTGAGTGAATTTTTTATTTGATATTGTTTTGAGACCTAGAAAATTTCTAAAGGGC
TCGAAGAAGTACCCGCTTTGCAAAAGGAAGTAACGACCATGACTATATCCGCTTTTTGGG
AAAGTAAGAGGTCAATGGAGACTAATCTATTATCTTTGTAGTGTGTGCTGCACCGGGTTC
CAAAACATTTCAGCTGCTTGAGATTATACATGAAGCATCAGAACCAGGATCTCTTCCTAA
TGGATTGGTTTGTGTTTCTATTTTATATCTACTATTTTCTAAATCTGATTTTAGGTAGAT
AGATAATTTGATTGTTCTTCCCTAAATAACCAGGTGGTGGCTAATGATGTTGATTTTAAA
AGATCTAACCTTCTCATTCACCAAACAAAGAGAATGTGCACATCCAACTTGATAGTGACA
AATCATGAAGGGCAACAGTTTCCTGGTTGCCGTTTGAACAAATCCCGAGCTTCTGAGAAA
GGAATAAGTGAAAATATGCCTATTAATCAACTTGCCTTTGACCGTGTTCTATGCGATGTT
CCGTGCAGTGGTGATGGTACACTGCGCAAGGCTCCAGATATCTGGCGCANATGGTATTTT
CACCTTTGGCATGTTTTACCTATTGGCAATGTTGTGAATGCATTATATTGTTTTTCTCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
   
Sequence Name 10-K110306-0022-590-A04C-8409
GenBank Accession LN490706 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K110306-0022-590-A04C-8409
GCCATTTACAATTGAATTATCCTGAGAAAAACAATATAATGCATTCACAACATTGCCAAT
AGGTAAAACATGCCAAAGGTGAAAATACCATTTGCGCCAGATATCTGGAGCCTTGCGCAG
TGTACCATCACCACTGCACGGAACATCGCATAGAACACGGTCAAAGGCAAGTTGATTAAT
AGGCATATTTTCACTTATTCCTTTCTCAGAAGCTCGGGATTTGTTCAAACGGCAACCAGG
AAACTGTTGCCCTTCATGATTTGTCACTATCAAGTTGGATGTGCACATTCTCTTTGTTTG
GTGAATGAGAAGGTTAGATCTTTTAAAATCAACATCATTAGCCACCACCTGGTTATTTAG
GGAAGAACAATCAAATTATCTATCTACCTAAAATCAGATTTAGAAAATAGTAGATATAAA
ATAGAAACACAAACCAATCCATTAGGAAGAGATCCTGGTTCTGATGCTTCATGTATAATC
TCAAGCAGCTGAAATGTTTTGGAACCCGGTGCAGCACACACTACAAAGATAATAGATTAG
TCTCCATTGACCTCTTACTTTCCCAAAAAGCGGATATAGTCATGGTCGTTACTTCCTTTT
GCAAAGCGGGTACTTCTTCGAGCCCTTTAGAAATTTTCTAGGTCTCAAAACAATATCAAA
TAAAAAATTCACTCACTGTCAAGTACGAAATGATCTGGATGTACGTCTAGGAAGAGAGGA
GGTACCTGCATG

Last Updated on 06.12.2018 16:11