Sequences from confirmation amplicon


Confirmation sequences for line 590B04 and hit At5g41180 are shown.
Line ID 590B04
Locus At5g41180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16484750
Primers used for PCR EU11 - 8474, show primer details
   
Sequence Name 06-K103353-0022-590-B04C-EU11
GenBank Accession LN488772 [GenBank]
Sequencing primer gene specific primer, EU11: 5'-ATTGGAATGGCATTAAATGTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103353-0022-590-B04C-EU11
AACTTTGATCTCTTTCTATCGTAAAATCAGTTATGTTTAGTATGATGATGATTTGGTATC
TGTTTCATGCTGTGAAACTTGCAGAAATATATCGGGGACATCGATGAGAGGGTTTCTTGT
GCCAGAACTTGGTCAAATAACCTACTTGCAAGAACTGTATGGTTTTGATTCATATTGACA
ATACCTGAAGATATAAGTTTGATGATTGGTACTGTTTGTAAATGTTTAGATGACTTTGTT
TTTTCTGTGTTGAATGCTTCTTTAGGATCCTGCGTGGGAACATTCTAATGGAAATCCAAT
TAATGGCTTCATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCACGGTT
   
Sequence Name 22-K103354-0022-590-B04C-8409
GenBank Accession LN496075 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K103354-0022-590-B04C-8409
CATTTACAATTGAATATATCATGAAGCCATTAATTGGATTTCCATTAGAATGTTCCCACG
CAGGATCCTAAAGAAGCATTCAACACAGAAAAAACAAAGTCATCTAAACATTTACAAACA
GTACCAATCATCAAACTTATATCTTCAGGTATTGTCAATATGAATCAAAACCATACAGTT
CTTGCAAGTAGGTTATTTGACCAAGTTCTGGCACAAGAAACCCTCTCATCGATGTCCCCG
ATATATTTCTGCAAGTTTCACAGCATGAAACAGATACCAAATCATCATCATACTAAACAT
AACTGATTTTACTGATAGAAAGAGATCAAAGTTTCCTACATCTTGATAATGTGATCCTTA
GATGGAGAACATTTAATGCCATTCCA
Primers used for PCR 35wq - 3144, show primer details
   
Sequence Name 23-K118742-0022-590-B04C-35wq
GenBank Accession LN496767 [GenBank]
Sequencing primer gene specific primer, 35wq: 5'-GTGACTATCTCAAAATTCCGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118742-0022-590-B04C-35wq
ACGGTTACGCTAAGCGAGCTAACCAATTGAAACAATTTCAAAATGAAAAACAAGATAAAG
AAACGATTATCAGCATCAAGAAAGTGAAACTCATATTCTACAAACCACATTCTGAAAGAG
GTCTCTGCTTAACATCCATGGTTTTCATGCAGTTTCCTTGAAAGCTCGTTCTACAATGAT
GTTCAATGATACAGAGAGATATTATCAAATGAGAAAAATAAAGGAAAGCTAGAAAAAGGG
TCTTATAAGAAATACATTGGGAGGTAATCCAAGCAACTCGGAACTCTTCCCTCGAAAAAG
TTGTAAGAGAAATCTGCCACTTTAAATAGAGAAGACTTGCACAAACCACTGATGTTTGCA
CTTGGATTTGAAGCATACCTGACCAACACAAAAAGAAGATACATTAACATTTCAATCATA
AACATTAAGTTTCCATCTTATGCTACCTTGGACTATTAGCTAAACTCACTTTTTTGATGT
TTTCGCGGCAATAGGAATACTTCCTCGAAGCCTATTCCTGCCAATAAGAAGTTCTTTAAG
GTGCTTCAAGTTTCCAATCTCTGGAGGTAACTTTCCTATTAAACCATTGGACTGAAGGTT
TCTGTGGTGTTAAAGAGACACAAAATGGTTATCTCAGCACATTATCGGATAGATGACATT
CTCTTAAAGATTTTCTTACATTGTCTTAATCCTTGACAATTTCCCGATCTCTGCTGGAAT
CGGTCCTGTCAAATGATTGTTTCCCAGGTCTAAGATCTTGAGTTTCTTTAACTTTCCTAT
CTCCTTTGGTATTCAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCC
CGGGTACCCTGTCCTCTCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAA
GGATAGTGGGATTGTGCGTC
   
Sequence Name 07-K118741-0022-590-B04C-35St
GenBank Accession LN489485 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118741-0022-590-B04C-35St
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTGAATACCAAAGGAGA
TAGGAAAGTTAAAGAAACTCAAGATCTTAGACCTGGGAAACAATCATTTGACAGGACCGA
TTCCAGCAGAGATCGGGAAATTGTCAAGGATTAAGACAATGTAAGAAAATCTTTAAGAGA
ATGTCATCTATCCGATAATGTGCTGAGATAACCATTTTGTGTCTCTTTAACACCACAGAA
ACCTTCAGTCCAATGGTTTAATAGGAAAGTTACCTCCAGAGATTGGAAACTTGAAGCACC
TTAAAGAACTTCTTATTGGCAGGAATAGGCTTCGAGGAAGTATTCCTATTGCCGCGAAAA
CATCAAAAAAGTGAGTTTAGCTAATAGTCCAAGGTAGCATAAGATGGAAACTTAATGTTT
ATGATTGAAATGTTAATGTATCTTCTTTTTGTGTTGGTCAGGTATGCTTCAAATCCAAGT
GCAAACATCAGTGGTTTGTGCAAGTCTTCTCTATTTAAAGTGGCAGATTTCTCTTACAAC
TTTTTCGAGGGAAGAGTTCCGAGTTGCTTGGATTACCTCCCAATGTATTTCTTATAAGAC
CCTTTTTCTAGCTTTCCTTTATTTTTCTCATTTGATAATATCTCTCTGTATCATTGAACA
TCATTGTAGAACGAGCTTTCAAGGAAACTGCATGAAAACCATGGATGTTAAGCAGAGACC
TCTNTCAGAATGTGGTTTGTAGAATATGAGTTTCACTTTCTTGATGCTGATAATCGTTTC
TTTATCTTGTTTTTCATTTTGAAATTNGTTTCATTGGTTAGCTCGCTTAGCTGTAACCGT

Last Updated on 06.12.2018 16:11