Sequences from confirmation amplicon


Confirmation sequences for line 592C03 and hit At3g58220 are shown.
Line ID 592C03
Locus At3g58220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21566434
Primers used for PCR CH53 - 8474, show primer details
   
Sequence Name 56-K105791-0022-592-C03C-CH53
GenBank Accession LN509105 [GenBank]
Sequencing primer gene specific primer, CH53: 5'-TTTATAACCCCAGGAAGGAGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K105791-0022-592-C03C-CH53
TGAATTTCCGAAACAACAACACTAGTCAAGCATCACACAATGATAACCATAAATACAGAC
ACAGAATCATAAAAATCTACACTTTTTTGTCCAGTGCAGTAGAAAAACTCACATTTCTGT
TGGGAGGGCTTATACAAATTGTGATTTACAACTTCCATGCGCAATTCTGTGTTTATACTC
CATCCAGAAGGCAAATGTTTAGAATCAGCAACTTCCACATACAAAGACATATACTTTTTA
ATCCTATTTCCCATGGGATAGGCTATAAGACGCCTGTAAGCAAAACAAATGAATATGTGT
CACATGAAACCCTAGCTAAAATTCACCGGAAAACATCAGAAAAAAAGACAGAGTGGTTTC
TTACCATTTGCAGCCACCGATCACAAATTCGTCAGAATAAATTGCCCTCACTCCCAAAGA
GGAGAAATCCTTGATTACCCAACTGAACTCGTTCTTACCAAGTGGACTCCCTATTCTCCC
CATTGAAGACTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TT
   
Sequence Name 64-K105794-0022-592-C03C-8409
GenBank Accession LN511206 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K105794-0022-592-C03C-8409
TGGGGAGAATAGGGAGTCCACTTGGTAAGAACGAGTTCAGTTGGGCTAATCAAGGATTTC
TCCTCTTTGGGAGTGAGGGCAATTTATTCTGACGAATTTGTGATCGGTGGCTGCAAATGG
TAAGAAACCACTCTGTCTTTTTTTCTGATGTTTTCCGGTGAATTTTAGCTAGGGTTTCAT
GTGACACATATTCATTTGTTTTGCTTACAGGCGTCTTATAGCCTATCCCATGGGAAATAG
GATTAAAAAGTATATGTCTTTGTATGTGGAAGTTGCTGATTCTAAACATTTGCCTTCTGG
ATGGAGTATAAACACAGAATTGCGCATGGAAGTTGTAAATCACAATTTGTATAAGCCCTC
CCAACAGAAATGTGAGTTTTTCTACTGCACTGGACAAAAAAGTGTAGATTTTTATGATTC
TGTGTCTGTATTTCTGTTTATCATTGTGTGATGCTTGACTAGTGTTGTTTGTTTCGTGAA
ATTTCAGATAGAAAAAATTTGTGGTTTGATCAGAAGACTCCTTCCTGGGGTTATAA

Last Updated on 06.12.2018 16:11