Sequences from confirmation amplicon


Confirmation sequences for line 592C03 and hit At4g29930 are shown.
Line ID 592C03
Locus At4g29930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14646808
Primers used for PCR 28uv - 8474, show primer details
   
Sequence Name 82-K117739-0022-592-C03C-28uv
GenBank Accession LN514234 [GenBank]
Sequencing primer gene specific primer, 28uv: 5'-GGCAGCACGTAAAAAGAAAATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K117739-0022-592-C03C-28uv
CTGCACGCATTACTACTATAATTATTTCCCGTGGAAAATGAGATTGGTGGATAAATCATT
GTTTAATGTTTTTGTATAATCTTTAAAAGGACGGATAAGGACAGGTTTGAAATGAAATGA
TCGACTTTTTATTTGATAAATGATTGGAGTATGATTTCACCAACTCTCAATGACTCCACT
GGTCTATACCTCACTGTAATTTTAGCGTCTGATTATATTATCTTTTATCAAGAAAATGTT
ATAAAGGTGCATAATTCTTTTTTGTCAAAGAAAACCAACATAATATTATATTCATTTGTG
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGT
TATTAT
   
Sequence Name 66-K117738-0022-592-C03C-8409
GenBank Accession LN511696 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K117738-0022-592-C03C-8409
TCGGACATGAGCCATTCACAAATGAATATATATTATGTTGGTTTTCTTTGACAAAAAAGA
ATTATGCACCTTTATAACATTTTCTTGATAAAAGATAATATAATCAGACGCTAAAATTAC
AGTGAGGTATAGACCAGTGGAGTCATTGAGAGTTGGTGAAATCATACTCCAATCATTTAT
CAAATAAAAAGTCGATCATTTCATTTCAAACCTGTCCTTATCCGTCCTTTTAAAGATTAT
ACAAAAACATTAAACAATGATTTATCCACCAATCTCATTTTCCACGGGAAATAATTATAG
TAGTAATGCGTGCAGGTGTATATACACAAATTTATCAGTTCTAATTTTCTTTTTACGTGC
TGCC

Last Updated on 06.12.2018 16:11