Sequences from confirmation amplicon


Confirmation sequences for line 593C11 and hit At4g36180 are shown.
Line ID 593C11
Locus At4g36180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17122861
Primers used for PCR EU12 - 8474, show primer details
   
Sequence Name 09-K103353-0022-593-C11C-EU12
GenBank Accession LN490138 [GenBank]
Sequencing primer gene specific primer, EU12: 5'-TCCGTCTTTCTTCAGTACAACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K103353-0022-593-C11C-EU12
GATCTCACCTCGCTTGAGGTCTTTAACGTCGCTGGAAACCGTCTCTCCGGTGAGATCCCC
GTTGGTTTACCATCGAGTCTCCAGTTCCTTGACATTTCGTCGAACACCTTCTCTGGTCAG
ATACCGAGTGGACTCGCTAACTTGACTCAGCTTCAGCTTCTTAATCTCTCGTATAATCAG
TTAACCGGCGAGATTCCGGCGAGTCTAGGTAACCTCCAAAGTCTTCAGTACTTATGGCTT
GATTTCAATCTCTTGCAAGGAACATTACCGTCTGCTATCTCAAACTGCTCCTCCTTAGTT
CACTTGAGCGCGTCGGAGAACGAAATCGGCGGCGTGATTCCCGCCGCGTACGGTGCTCTT
CCGAAACTGGAGGTTCTCTCGCTCTCCAATAACAATTTTTCCGGTACTGTGCCGTTTTCT
CTCTTCTACATGTACTCAGGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 25-K103354-0022-593-C11C-8409
GenBank Accession LN497400 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K103354-0022-593-C11C-8409
TTTACATTGAAATCCTGAGTACTGTAGAAGAGAGAAAACGGCACAGTACCGGAAAAATTG
TTATTGGAGAGCGAGAGAACCTCCAGTTTCGGAAGAGCACCGTACGCGGCGGGAATCACG
CCGCCGATTTCGTTCTCCGACGCGCTCAAGTGAACTAAGGAGGAGCAGTTTGAGATAGCA
GACGGTAATGTTCCTTGCAAGAGATTGAAATCAAGCCATAAGTACTGAAGACTTTGGAGG
TTACCTAGACTCGCCGGAATCTCGCCGGTTAACTGATTATACGAGAGATTAAGAAGCTGA
AGCTGAGTCAAGTTAGCGAGTCCACTCGGTATCTGACCAGAGAAGGTGTTCGACGAAATG
TCAAGGAACTGGAGACTCGATGGTAAACCAACGGGGATCTCACCGGAGAGACGGTTTCCA
GCGACGTTAAAGACCTCAAGCGAGGTGAGATTCCTCATCGCCGGAGGTAGTTTACCGGAG
AGTGAGTTGTACTGAAAAAAGACGGA

Last Updated on 06.12.2018 16:11