Sequences from confirmation amplicon


Confirmation sequences for line 594D07 and hit At1g08590 are shown.
Line ID 594D07
Locus At1g08590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2719206
Primers used for PCR EU14 - 8474, show primer details
   
Sequence Name 15-K103353-0022-594-D07C-EU14
GenBank Accession LN492935 [GenBank]
Sequencing primer gene specific primer, EU14: 5'-AATGGAACTCGCATATTATTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103353-0022-594-D07C-EU14
AATATTGAGAGAGATTAAATAAAATTAGATTCTGACTAATCATTGTTTTTCAGCTTTTGG
TTTCCACTCTCTTATTATCAACTTCGCTTGGGGACCTTCAATCTCAGTTGCACAAAAATG
CCATAAAACTAGCCAAAAAGTGAAGAAATAGAGGAGAAAAATGGCGATCCCTCGACTTTT
CTTCCTCTTCTATTACATTGGTTTCGCTCTTTTCCCTTTTGTTTCTTCTGAAACATTTCA
AAACTCCGAACAAGAGATCTTGTTGGCCTTCAAGTCAGACCTTTTTGATCCTTCAAATAA
TCTCCAAGATTGGAAGAGACCTGAGAATGCCACAACATTTTCTGAGTTGGTTCATTGTCA
TTGGACAGGAGTTCACTGTGATGCAAATGGCTATGTGGCTAAGCTTCTTCTCTCCAACAT
GAATCTCAGCGGAAACGTGTCAGATCAGATTCAGAGCTTCCCTTCTCTGCAAGCCCTTGA
TCTCAGCAATAACGCATTTGAGTCTTCTCTTCCTAATCTAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 31-K103354-0022-594-D07C-8409
GenBank Accession LN500125 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103354-0022-594-D07C-8409
TTTCATTAGATTAGGAAGAGAGACTCAAATGCGTTATTGCTGAGATCAAGGGCTTGCAGA
GAAGGGAAGCTCTGAATCTGATCTGACACGTTTCCGCTGAGATTCATGTTGGAGAGAAGA
AGCTTAGCCACATAGCCATTTGCATCACAGTGAACTCCTGTCCAATGACAATGAACCAAC
TCAGAAAATGTTGTGGCATTCTCAGGTCTCTTCCAATCTTGGAGATTATTTGAAGGATCA
AAAAGGTCTGACTTGAAGGCCAACAAGATCTCTTGTTCGGAGTTTTGAAATGTTTCAGAA
GAAACAAAAGGGAAAAGAGCGAAACCAATGTAATAGAAGAGGAAGAAAAGTCGAGGGATC
GCCATTTTTCTCCTCTATTTCTTCACTTTTTGGCTAGTTTTATGGCATTTTTGTGCAACT
GAGATTGAAGGTCCCCAAGCGAAGTTGATAATAAGAGAGTGGAAACCAAAAGCTGAAAAA
CAATGATTAGTCAGAATCTAATTTTATTTAATCTCTCTCAATTATTTTATCATGATTGTG
TTTCTTTTTCGTTCGGTGGAATAATATGCG
Primers used for PCR EU15 - 8474, show primer details
   
Sequence Name 34-K103433-0022-594-D07C-EU15
GenBank Accession LN501381 [GenBank]
Sequencing primer gene specific primer, EU15: 5'-AGCCATTGTAACCAAGAATAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K103433-0022-594-D07C-EU15
TTCGGACCTTTCCACCAAGTTGTTACCCGAAAGACCGAGAAACTTCAGATTCTTGAGATT
CTTGAAAGAAGAAGGCACTGAGCCCTCGAAGTAACCTCCTCGAAAGTCCAAAACCTCAAG
TGTAGTTGCGTTGCCAAGATCTTCAGGTAGAAACCCCGAAAAATTGTTGCTTGAGGCATT
GACGTGTGTAAGACCAGTTGCCATTCCTAGACCATAAGGGAAGGTTCCAAAGAAGCTGTT
CACACTCACATCAATGACCTTCAAAGACGTGAGATTAGAAAGAGATTTAGGAAGAAAAAG
AAAAAGAAAGAGTAATGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 50-K103434-0022-594-D07C-8409
GenBank Accession LN507135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K103434-0022-594-D07C-8409
GCCATTTACAATTGAATAATACTCATTACTCTTTCTTTTTCTTTTTCTTCCTAAATCTCT
TTCTAATCTCACGTCTTTGAAGGTCATTGATGTGAGTGTGAACAGCTTCTTTGGAACCTT
CCCTTATGGTCTAGGAATGGCAACTGGTCTTACACACGTCAATGCCTCAAGCAACAATTT
TTCGGGGTTTCTACCTGAAGATCTTGGCAACGCAACTACACTTGAGGTTTTGGACTTTCG
AGGAGGTTACTTCGAGGGCTCAGTGCCTTCTTCTTTCAAGAATCTCAAGAATCTGAAGTT
TCTCGGTCTTTCGGGTAACAACTTTGGTGGAAAGGTACCGAAGGTGATTGGTGAGCTCTC
TTCCTTGGAGACCATTATTCTTGGTTACAATGG

Last Updated on 06.12.2018 16:11