Sequences from confirmation amplicon


Confirmation sequences for line 594E02 and hit At5g49430 are shown.
Line ID 594E02
Locus At5g49430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20038018
Primers used for PCR 29uv - 8474, show primer details
   
Sequence Name 83-K117671-0022-594-E02C-29uv
GenBank Accession LN514352 [GenBank]
Sequencing primer gene specific primer, 29uv: 5'-CTGCAGAATGAATCCATAGAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K117671-0022-594-E02C-29uv
CTCTTCAGGGTTGTAAAATTTACCTGTTGGAGTAGCTGGAATTTCCCACGGTCTGGTTCA
GTTTTCGGAGGCCAAAAGAATCCTGAAACAAATTAGCCCACATTATATCAATATTGTTAG
TTCTTGGGATAATGTTGTGTCTTAATCCACTAAGCTTTAGAATGTGATCTATAAACCAAC
TAAGCTAATGTAAACATGGGCCTAAGCTCTTTGGGTGAAGTCTGAATTTGGTGGGGAGGA
TAGGCTAGATTTAACACCATGAGATATTATATTCATTAGGATCACATACCTGGGTTCTCT
TGTCTGAAGTCTCTAACTTTGTTAAGGCCGAGAGCAAACGGTTTCGTTGCTCATCATCAA
TGTGAGGCTGCTCCCACTTGGTATCTGCATCAAAAAGCTCCCAGGGACTGTGAAGATGTG
TCTCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 35-K117670-0022-594-E02C-8409
GenBank Accession LN501986 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117670-0022-594-E02C-8409
ATTTCATTGAATATTCCTGCCGAGACACATCTTCACAGTCCCTGGGAGCTTTTTGATGCA
GATACCAAGTGGGAGCAGCCTCACATTGATGATGAGCAACGAAACCGTTTGCTCTCGGCC
TTAACAAAGTTAGAGACTTCAGACAAGAGAACCCAGGTATGTGATCCTAATGAATATAAT
ATCTCATGGTGTTAAATCTAGCCTATCCTCCCCACCAAATTCAGACTTCACCCAAAGAGC
TTAGGCCCATGTTTACATTAGCTTAGTTGGTTTATAGATCACATTCTAAAGCTTAGTGGA
TTAAGACACAACATTATCCCAAGAACTAACAATATTGATATAATGTGGGCTAATTTGTTT
CAGGATTCTTTTGGCCTCCGAAAACTGAACCAGACCGTGGGAAATTCCAGCTACTCCAAC
AGGTAAATTTCTACAACCCTGGAAGAGTCTTTGGTGGAGCCAAAGCTGACATGGTTTTGT
TCTATGGATTCATTCTGCA

Last Updated on 06.12.2018 16:11