Sequences from confirmation amplicon


Confirmation sequences for line 595B03 and hit At1g10390 are shown.
Line ID 595B03
Locus At1g10390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3410069
Primers used for PCR CD88 - 8474, show primer details
   
Sequence Name 36-K105634-0022-595-B03C-CD88
GenBank Accession LN502225 [GenBank]
Sequencing primer gene specific primer, CD88: 5'-GAAGTTGTTGAAACCAAAACCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K105634-0022-595-B03C-CD88
AGGGTGTCCACCAGCAGGCTACAGAAATAATGCGAGATTTGAAGGCCAAAATTAGAAAAG
AAGGAAACTAGGTGCCGGTGTCATTTAAGAGATGCAAAAGAACTGCAAACTATGAAGAGA
ACAGGCATCACCAAAAGAAATGAAAAAAAAAAAAAAAACAAGGGGCTTGGGCTTAAAACC
AAGGGGAAAATCCCCCTTTTTTTGCCCAAGGGGAAACCTCCAAACCGGGTTTATTTTTAA
AAAACGGAAAAACTTTTTCCCAATCCCGGGGAAGGGTTTTTAAAAATGGCTTTTTGGCCC
AATGGCGGGACCCCTACTTTGCCCAAATTGCCAAAAACAAAAACCTTTTTTTCAAAACCA
AAAACCATTCTCCCGGGCAAAGGGGTTTAATTTAAACAAAAAAAAGGGCTAATGGTTCCC
AATGGCGGAATTTCCCCCCGGCCCGGGACTTAACCCAAAATTTGTTAAAAAAAACCCAAT
CCCGGGGGGGTTTGAAAACCCAAAAACGGGGGAGGGGGTCTTCCCAAACCATTTGGGAAA
AAAATTTTAACCCCAGGGCCCTTGAAAAGGGCTTCTTGCCGGGAAATTCCCCGGGGTCCC
CCAGGGATTTGGAGGGCCAATTGGGAAAAAAAAAAAAATAATTTCCCATTTTTTTTCCAT
TTCAAAAAGGAAAATTCCCCCCCCGTTTTTAAA
   
Sequence Name 52-K105635-0022-595-B03C-8409
GenBank Accession LN507818 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K105635-0022-595-B03C-8409
ATTTACATGGCCATTGGTATCATACTCTTTCTCCATATTGTTTTGGAACGACACCATCAC
CGTTATTTGGTTCATCAAGCACTCCTGGATTTGGCTCTTCTTCATCAATCTTTGGTTCAG
CTCCTGGTCAGGGGGCAACTCCAGCATTTGGCAACAGTCAGCCATCTACTCTGTTCAATT
CAACCCCTTCGACCGGGCAGACTGGTTCTGCGTTTGGACAGACTGGTTCTGCTTTTGGAC
AGTTCGGACAGAGTAGTGCTCCTGCATTTGGCCAAAATAGCATTTTTAATAAACCTTCCA
CTGGATTTGGAAACATGTTTTCCAGTTCTTCAACATTAACCACGTCTAGCAGCTCTCCCT
TTGGACAAACAATGGTGATTTTCACATTCGCTATTAAGCCTAAGCACTTCGTTTTTTTTT
TTTTTTCCTTTTCTTTGGGGAAGCCCGGTTCCCTCAAAATTTGGAATTTTTTTGGCATCC
CTAAAAAAACCCCGCCCCCAATTTCCCTTTTTTTAAAATTTGGCCCTAAAAACCCCCATT
TTTTTTGGAACCCGGGGGGGGGAACCCCTTTTAAATCCCCTCACCCGGGTAAACCATAAA
AGGTTTTGGTTTCAAAAAATTAA
Primers used for PCR 66wf - 8474, show primer details
   
Sequence Name 37-K118301-0022-595-B03C-66wf
GenBank Accession LN502779 [GenBank]
Sequencing primer gene specific primer, 66wf: 5'-TAACTGCTAATTGGTGTGTTGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118301-0022-595-B03C-66wf
CCCCTACATTTGGGGGTTCTGGTTTTGGTCAATCAACTTTCGGTGGTCAACAAGGGGGCA
GTCGAGCTGTGCCTTATGCACCAACTGTTGAGGCAGACACAGGGACCGGCACTCAGCCTG
CTGGAAAGTTGGAATCTATTTCTGCCATGCCAGCATACAAAGAGAAAAACTATGAGGAGC
TGAGGTGGGAAGATTACCAGCGAGGAGATAAAGGTACACCTGATCAAACAAATCTTGTAC
TACATCTGCTTAAGGCTACATTCCTGTGCCTTATTTAACTAAATCTTGATTCTCGTACTC
TTCAATTCTCAGGTGGACCACTTCCTGCTGGCCAGTCTCCTGGCAATGCCGGATTTGGTA
TATCACCTTCTCAACCAAATCCATTTTCTCCATCACCGGCGTTTGGTCAGACATCAGCAA
ATCCAACAAACCCTTTTTCGAGCTCAACATCTACCAACCCTTTTGCCCCTCAAACCCCAA
CAATTGCTAGTTCGAGTTTTGGAACGGCTACCTCAAATTTTGGTTCCTCGCCCTTTGGTG
TAACATCTTCTTCCAATCTTTTTGGTTCAATAAGGATATATAGGCAAATAATTAGTAATT
ACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGC
   
Sequence Name 05-K118300-0022-595-B03C-8409
GenBank Accession LN488551 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118300-0022-595-B03C-8409
ATTTCATTGAATATATCCTGGTAATTACTAATTATTTGCCTATATATCCTTATTGAACCA
AAAAGATTGGAAGAAGATGTTACACCAAAGGGCGAGGAACCAAAATTTGAGGTAGCCGTT
CCAAAACTCGAACTAGCAATTGTTGGGGTTTGAGGGGCAAAAGGGTTGGTAGATGTTGAG
CTCGAAAAAGGGTTTGTTGGATTTGCTGATGTCTGACCAAACGCCGGTGATGGAGAAAAT
GGATTTGGTTGAGAAGGTGATATACCAAATCCGGCATTGCCAGGAGACTGGCCAGCAGGA
AGTGGTCCACCTGAGAATTGAAGAGTACGAGAATCAAGATTTAGTTAAATAAGGCACAGG
AATGTAGCCTTAAGCAGATGTAGTACAAGATTTGTTTGATCAGGTGTACCTTTATCTCCT
CGCTGGTAATCTTCCCACCTCAGCTCCTCATAGTTTTTCTCTTTGTATGCTGGCATGGCA
GAAATAGATTCCAACTTTCCAGCAGGCTGAGTGCCGGTCCCTGTGTCTGCCTCAACAGTT
GGTGCATAAGGCACAGCTCGACTGCCCCCTTGTTGACCACCGAAAGTTGATTGACCAAAA
CCAGAACCCCCAAATGTAGGGGTTGAAGCCTGCGCACCTGCAAAACAAATCATGCAAACA
TATTTCGTCACCCCTTTATTCAAATGAGCAACACACCAATAAAGCAGTT

Last Updated on 06.12.2018 16:11