Sequences from confirmation amplicon


Confirmation sequences for line 595B03 and hit F22J12 are shown.
Line ID 595B03
Locus F22J12 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15498813
Primers used for PCR 37we - 3144, show primer details
   
Sequence Name 36-K118301-0022-595-B03C-37we
GenBank Accession LN502397 [GenBank]
Sequencing primer gene specific primer, 37we: 5'-GATTATTCCTGCTGAAAGTTCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118301-0022-595-B03C-37we
GGGGGAGAGTGTCGAGAGATTGGAGATTTGAAGCCATGGGATCTTGAGTCAGCTTCGTGG
TTTGTTCTCCCATACATGTCCTCTGTCTTTCTCTTATAGGGGTTCAGCGCCTCTTTGTTT
CTCGAAAAATCTGAAAGGTGAGACGACCCATTTTCCTGAGATAACCTGAGGCCTTGATTG
TTAGATCTGTGGCCTTCCTTTGGTTTTTGAGCTGTTCTCTCAGGGGGAACTTGATTTGTC
CTTTTATTTGTCTCGTCAGCTTTTTTAAGGCCAGGGCAATCAACTTCTACATGAGATAAG
CGGAGACAATGGTGGCAATGGCTTTTCAAATTTTTGTAGTCCAGACTAACTACAACTTCA
CTACCATCTTGGAACTCCACTGTTGTTTCTTTTGTAAGAGGTTGTAAACCGTTGATTAAG
ACTCTTATTTTAGCTGCAGATGGGGAGATATCACATCAATCCAC
   
Sequence Name 04-K118300-0022-595-B03C-35St
GenBank Accession LN488110 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118300-0022-595-B03C-35St
CGGTTACACCTCTTAAAAGAAACACAGTGGAGTTCCAAGATGGTAGTGAAGTTGTAGTTA
GTCTGGACTACAAAAATTTGAAAAGCCATTGCCACCATTGTCTCCGCTTATCTCATGTAG
AAGTTGATTGCCCTGGCCTTAAAAAAGCTGACGAGACAAATAAAAGGACAAATCAAGTTC
CCCCTGAGAGAACAGCTCAAAAACCAAAGGAAGGCCACAGATCTAACAATCAAGGCCTCA
GGTTATCTCAGGAAAATGGGTCGTCTCACCTTTCAGATTTTTCGAGAAACAAAGAGGCGC
TGAACCCCTATAAGAGAAAGACAGAGGACATGTATGGGAGAACAAACCACGAAGCTGACT
CAAGATCCCATGGCTTCAAATCTCCAATCTCTCGACACTCTCCCCCTCGACACACTCCTT
CTTTCAGAAGAGAACCTCCCAGAACTTTCAGCAGGAATAATCATC

Last Updated on 06.12.2018 16:11