Sequences from confirmation amplicon


Confirmation sequences for line 596C01 and hit At5g11150 are shown.
Line ID 596C01
Locus At5g11150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3547154
Primers used for PCR MN26 - 8474, show primer details
   
Sequence Name 77-K104224-0022-596-C01C-MN26
GenBank Accession LN513544 [GenBank]
Sequencing primer gene specific primer, MN26: 5'-CTAGCTCAATCTCGAAGCAGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K104224-0022-596-C01C-MN26
CGATGCCATATGTCTTACTCTCAGGATCGCTACATCTTCCATGTGAAACGAACAGATGGT
CTTACCGTTCTCTGTATGGCCGACGAAACCGCCGGAAGTAAGCTGCTTCTCTCGCTAATT
TCATCAATTTTACATGATTTGGCTCTTGTATTTGAGTGTCTTCGATTTCTGCTGAGATCT
TATATCAATTCTGGGGGATTTGATTGTTGTTTCAATAGAAAGATGCTGATAATTTTCTGG
GAGCAATTTGGTGATGTTAGGTTTATGTCACTTATGATTTTGCTCTGATGTTGCGGCAGG
GAATATTCCGTTTGCGTTTTTGGATGATATTCATCAGAGGTTTGTGAAAACATATGGCCG
AGCTATTCATTCAGCTCAAGCTTATTCCATGAATGATGAATTTTCAAGAGTTCTGAGTCA
ACAGATGGAGTTTTATTCCAATGATCCTAATGCAGACAGGATGAGCAGAATCAAAGGTGA
AATGAGTCAGGTAATGTTACAAAATCATCTCTTTTTTGAAGTCTAAAGTATATGTTTGAT
ATAGCCTATTACGACACATACATGGACAATAAACATTGCTGATCCATGTAAATGGCTTCA
TGTCCGGGATCATCATCGACGACGGATATGTTATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 37-K104225-0022-596-C01C-8409
GenBank Accession LN502600 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K104225-0022-596-C01C-8409
TTACATAACATATCCGTCGTCGATGATGATCCCGGACATGAAGCCATTTACATGGATCAG
CAATGTTTATTGTCCATGTATGTGTCGTAATAGGCTATATCAAACATATACTTTAGACTT
CAAAAAAGAGATGATTTTGTAACATTACCTGACTCATTTCACCTTTGATTCTGCTCATCC
TGTCTGCATTAGGATCATTGGAATAAAACTCCATCTGTTGACTCAGAACTCTTGAAAATT
CATCATTCATGGAATAAGCTTGAGCTGAATGAATAGCTCGGCCATATGTTTTCACAAACC
TCTGATGAATATCATCCAAAAACGCAAACGGAATATTCCCTGCCGCAACATCAGAGCAAA
ATCATAAGTGACATAAACCTAACATCACCAAATTGCTCCCAGAAAATTATCAGCATCTTT
CTATTGAAACAACAATCAAATCCCCCAGAATTGATATAAGATCTCAGCAGAAATCGAAGA
CACTCAAATACAAGAGCCAAATCATGTAAAATTGATGAAATTAGCGAGAGAAGCAGCTTA
CTTCCGGCGGTTTCGTCGGCCATACAGAGAACGGTAAGACCATCTGTTCGTTTCACATGG
AAGATGTAGCGATCCTGAGAGTAAGACATATGGCTATCGCTATCGTTTCCAGGCAACTTC
TCCAAAATCT
Primers used for PCR CL37 - 8474, show primer details
   
Sequence Name 78-K104224-0022-596-C01C-CL37
GenBank Accession LN513684 [GenBank]
Sequencing primer gene specific primer, CL37: 5'-GAACAGATGGTCTTACCGTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K104224-0022-596-C01C-CL37
GCTGCTTCTCTCGCTAATTTCATCAATTTTACATGATTTGGCTCTTGTATTTGAGTGTCT
TCGATTTCTGCTGAGATCTTATATCAATTCTGGGGGATTTGATTGTTGTTTCAATAGAAA
GATGCTGATAATTTTCTGGGAGCAATTTGGTGATGTTAGGTTTATGTCACTTATGATTTT
GCTCTGATGTTGCGGCAGGGAATATTCCGTTTGCGTTTTTGGATGATATTCATCAGAGGT
TTGTGAAAACATATGGCCGAGCTATTCATTCAGCTCAAGCTTATTCCATGAATGATGAAT
TTTCAAGAGTTCTGAGTCAACAGATGGAGTTTTATTCCAATGATCCTAATGCAGACAGGA
TGAGCAGAATCAAAGGTGAAATGAGTCAGGTAATGTTACAAAATCATCTCTTTTTTGAAG
TCTAAAGTATATGTTTGATATAGCCTATTACGACACATACATGGACAATAAACATTGCTG
ATCCATGTAAATGGCTTCATGTCCGGGATCATCATCGACGACGGATATGTTATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 38-K104225-0022-596-C01C-OriL
GenBank Accession LN502963 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104225-0022-596-C01C-OriL
GCCTTACATAACATATCCGTCGTCGATGATGATCCCGGACATGAAGCCATTTACATGGAT
CAGCAATGTTTATTGTCCATGTATGTGTCGTAATAGGCTATATCAAACATATACTTTAGA
CTTCAAAAAAGAGATGATTTTGTAACATTACCTGACTCATTTCACCTTTGATTCTGCTCA
TCCTGTCTGCATTAGGATCATTGGAATAAAACTCCATCTGTTGACTCAGAACTCTTGAAA
ATTCATCATTCATGGAATAAGCTTGAGCTGAATGAATAGCTCGGCCATATGTTTTCACAA
ACCTCTGATGAATATCATCCAAAAACGCAAACGGAATATTCCCTGCCGCAACATCAGAGC
AAAATCATAAGTGACATAAACCTAACATCACCAAATTGCTCCCAGAAAATTATCAGCATC
TTTCTATTGAAACAACAATCAAATCCCCCAGAATTGATATAAGATCTCAGCAGAAATCGA
AGACACTCAAATACAAGAGCCAAATCATGTAAAATTGATGAAATTAGCGAGAGAAGCAGC
TTACTTCCGGCGGTTTCGTCGGCCATACAGAGAAC

Last Updated on 06.12.2018 16:11