Sequences from confirmation amplicon


Confirmation sequences for line 596C03 and hit At5g42280 are shown.
Line ID 596C03
Locus At5g42280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16906855
Primers used for PCR 20ak - 8474, show primer details
   
Sequence Name 52-K106747-0022-596-C03C-20ak
GenBank Accession LN507832 [GenBank]
Sequencing primer gene specific primer, 20ak: 5'-TCATCGAGAACAAAATCACACTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K106747-0022-596-C03C-20ak
TGGAAGGTGCTGCCTCACATACCTTGTCTTTCTCACAAATATTGTTCTTTTCATAAAGCT
TTAGATAATGCTCATGGCTGAAATGTTGAATTGTCTCCTCATCAATCCTAACAAATGACT
CCATATATTCCTCGGGTTCTTCAGGCACTCCTTCAAGATCTTTTCCATCCCAAACCTCTT
TTCTCGTTGCACATTTTGAATGGACAGCATAATGACATCCCTTATTACAAGAGTATTGCC
CGTTGTTGACGTCGATGGTTTGGCGACAGACTCCACATAAGAAATCACCAGGTTGAAGGG
ATAAACTGAAAGAGAGACGGTGTGGGTGTCGGGTGATCTTAATGACACGAGGTAGATAGA
TACATGATCTGTGGACCATGTGGCTGCAAGGGAGACAAGCGTAAACAAGATCATCGGTCT
CGCTTAGAGACAAGCCACATGCATCACAAGGAAAAGGAAGTCGTATAGGGAAGAGGGTGA
GGGTGTGCTCATGACTTTTGATGTGTGAGATAGTGGGAGGTGGTGGTCT
   
Sequence Name 46-K106746-0022-596-C03C-8409
GenBank Accession LN505873 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K106746-0022-596-C03C-8409
TGCTCGATGAGACCACCACCTCCCACTATCTCACACATCAAAAGTCATGAGCACACCCTC
ACCCTCTTCCCTATACGACTTCCTTTTCCTTGTGATGCATGTGGCTTGTCTCTAAGCGAG
ACCGATGATCTTGTTTACGCTTGTCTCCCTTGCAGCCACATGGTCCACAGATCATGTATC
TATCTACCTCGTGTCATTAAGATCACCCGACACCCACACCGTCTCTCTTTCAGTTTATCC
CTTCAACCTGGTGATTTCTTATGTGGAGTCTGTCGCCAAACCATCGACGTCAACAACGGG
CAATACTCTTGTAATAAGGGATGTCATTATGCTGTCCATTCAAAATGTGCAACGAGAAAA
GAGGTTTGGGATGGAAAAGATCTTGAAGGAGTGCCTGAAGAACCCGAGGAATATATGGAG
TCATTTGTTAGGATTGATGAGGAGACAATTCAACATTTCAGCCATGAGCATTATCTAAAG
CTTTATGAAAAGAACAATATTTGTGAGAAAGACAAGGTATGTGAGGCATGCACCCTTCCA
GTAATGATCTCTCAGAGATATTATGGTTGTATGAAGTGTGATTTTGTTCTCGATGA
Primers used for PCR 01am - 8474, show primer details
   
Sequence Name 41-K118466-0022-596-C03C-01am
GenBank Accession LN504258 [GenBank]
Sequencing primer gene specific primer, 01am: 5'-ATGATGCCTCTAAAAATGAATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118466-0022-596-C03C-01am
GTGATAGTGCCTGTTTATCTGATGGAAATCACCTGAAATGCAAATGTTGTCAGGTTCCAC
TACAAAAGGTATATTATCATTGTTCTATCTGCAAGTTCTATCTTAATGCGACTTGCTCGA
CAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CC
   
Sequence Name 14-K118465-0022-596-C03C-oriL
GenBank Accession LN492720 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118465-0022-596-C03C-oriL
TTTGTCGAGCAAGTCGCATTAAGATAGAACTTGCAGATAGAACAATGATAATATACCTTT
TGTAGTGGAACCTGACAACATTTGCATTTCAGGTGATTTCCATCAGATAAACAGGCACTA
TCACCATAATCATCATCACCATCACCATCATTGTCATTACTAACACCACTAGCGACGGCA
TTACTGTTGACGCTTTCTTCGTAGTCATCATCAACTTTGTATACCAATTCATTTTAGAAG
GCATC
Primers used for PCR 67wn - 8474, show primer details
   
Sequence Name 13-K118465-0022-596-C03C-8409
GenBank Accession LN492257 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118465-0022-596-C03C-8409
ATTTACATTTGTCGAGCAAGTCGCATTAAGATAGAACTTGCAGATAGAACAATGATAATA
TACCTTTTGTAGTGGAACCTGACAACATTTGCATTTCAGGTGATTTCCATCAGATAAACA
GGCACTATCACCATAATCATCATCACCATCACCATCATTGTCATTACTAACACCACTAGC
GACGGCATTACTGTTGACGCTTTCTTCGTAGTCATCATCAACTTTGTATACCAATTCATT
TTTAGAGGCATCATCATCGTTGTTGATGCTGAGTTCCAAAGCCCATGTCGAATAATCATT
ATTGTGGTCATCATCATCATCATCATCATCATCATCCTCATCCTCATCATCCTCATCCTC
ATCCTCATCATCATCATCTTCATCATCTTCATCATCAACAACAACATCATCATCATCATC
ATCATCATCATCATCATCATCAACAACATCATCATCGTTATCATCATCATCATCATCATC
ATCATCATCATCATCATTATTATTATTATCGTCATTACTATCAGCCTCAACATTATTGTT
GACATCGTCTTCGTAGACAACATTATTGTTGACGTCGTCTTCGTAGTCCTTATCAAATTC
GTGTACCGCTTCTTCACGAGTAGAAGCACCATTGGATAAAGAGTCACCATCACCATCATC
AGCAGCAACATCACTATTGTTGTCGTCGTCTTCACAATCATCATCAACTTCATATATTGC
TTCTGCTGAAGAGGACTTGTCTTTCCAGTTTCGGGATGAAAAATCAGAAGTCGGATCTAC

Last Updated on 06.12.2018 16:11