Sequences from confirmation amplicon


Confirmation sequences for line 596E10 and hit At3g46520 are shown.
Line ID 596E10
Locus At3g46520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17129789
Primers used for PCR 15ie - 8474, show primer details
   
Sequence Name 55-K112758-0022-596-E10C-15ie
GenBank Accession LN508860 [GenBank]
Sequencing primer gene specific primer, 15ie: 5'-GTTCACTTTGGTTCGATTTCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K112758-0022-596-E10C-15ie
TTTTGGACTCTGGAGATGGTGTGAGCCACCGGTACCAATCTACGAGGGTTATGCACTTCC
ACACGCAATCCTGCGTCTTGATCTTGCAGGTCGTGACCTAACCGACCACCTCATGAAAAT
CCTGACAGAGCGTGGTTACTCATTCACCACAACTGCTGAGCGTGAGATTGTCAGAGACAT
GAAGGAGAAGCTCTCGTACATTGCCTTGGACTATGAGCAAGAGCTTGAGACTTCCAAAAC
CAGCTCATCTGTGGAGAAGAGCTTCGAGCTGCCAGACGGTCAAGTGATAACCATCGGGGC
AGAGCGTTTCCGGTGTCCTGAAGTTCTGTTCCAGCCATCCATGATCGGAATGGAAAATCC
GGGAATTCATGAAACTACTTACAACTCAATCATGAAATGTGATGTGGATATCAGGAAGGA
TCTTTATGGAAACATTGTGCTTAGTGGTGGCACCACAATGTTTGGCGGGATTGGTGATAG
GATGAGTAAAGAAATCACCGCGTTGGCGCCGAGCAGTATGAAGATCAAAGTGGTTGCTCC
ACCGGAAAGGAAGTACAGTGTTTGGATCGGTGGCTCTATCTTGGCTTCTCTCAGTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 15-K112757-0022-596-E10C-8409
GenBank Accession LN493044 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112757-0022-596-E10C-8409
AAACAGATGAGCCCCGATCCAACACTGTACTTCCTTTCCGGTGGAGCAACCACTTTGATC
TTCATACTGCTCGGCGCCAACGCGGTGATTTCTTTACTCATCCTATCACCAATCCCGCCA
AACATTGTGGTGCCACCACTAAGCACAATGTTTCCATAAAGATCCTTCCTGATATCCACA
TCACATTTCATGATTGAGTTGTAAGTAGTTTCATGAATTCCCGGATTTTCCATTCCGATC
ATGGATGGCTGGAACAGAACTTCAGGACACCGGAAACGCTCTGCCCCGATGGTTATCACT
TGACCGTCTGGCAGCTCGAAGCTCTTCTCCACAGATGAGCTGGTTTTGGAAGTCTCAAGC
TCTTGCTCATAGTCCAAGGCAATGTACGAGAGCTTCTCCTTCATGTCTCTGACAATCTCA
CGCTCAGCAGTTGTGGTGAATGAGTAACCACGCTCTGTCAGGATTTTCATGAGGTGGTCG
GTTAGGTCACGACCTGCAAGATCAAGACGCAGGATTGCGTGTGGAAGTGCATAACCCTCG
TAGATTGGTACCGTGTGGCTCACACCATCTCCAGAGTCCAAAACAATACCTAACCAACAA
AATAAAAACAATGAGAGCAAATGAAATCGAACANAAGTGA

Last Updated on 06.12.2018 16:11