Sequences from confirmation amplicon


Confirmation sequences for line 596G04 and hit At1g78090 are shown.
Line ID 596G04
Locus At1g78090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29376111
Primers used for PCR JV58 - 8474, show primer details
   
Sequence Name 71-K104216-0022-596-G04C-JV58
GenBank Accession LN512553 [GenBank]
Sequencing primer gene specific primer, JV58: 5'-TAAAATCAGGTACTTGAAATCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K104216-0022-596-G04C-JV58
GCCGGCACTCAATTTTTTACTAAAATCATTAGGTGAGAGAGCCCCTCATAAATCTTATTT
ATTTTTCATTTCGATATGAGAATTTAAAGATCTCAAGTTCTCATATCATTTATCGACTAA
TTAAAATAGGATATGAAAATTCGGATGATGTTGTGCCGGTGTATATCGGGGATGACCGTA
CTGACGAAGATGCGTTTAAGGTATAGTGACACTTAACTTATACGTATTCGTAACATCAGT
TAATTAAGTCTTTTTACATTTTGAACAATTTGATTCTATTACTGACGTAATAAATGAACT
GTGTAATTCGATAGGTTTTACGTGAAAGGGGACAAGGTTTTGGGATTCTTGTCTCAAAAG
TACCAAAGGACACCAATGCCTCTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 31-K104217-0022-596-G04C-8409
GenBank Accession LN500143 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104217-0022-596-G04C-8409
TTAAATTGAATAAGAGGCATTGGTGTCCTTTGGTACTTTTGAGACAAGAATCCCAAAACC
TTGTCCCCTTTCACGTAAAACCTATCGAATTACACAGTTCATTTATTACGTCAGTAATAG
AATCAAATTGTTCAAAATGTAAAAAGACTTAATTAACTGATGTTACGAATACGTATAAGT
TAAGTGTCACTATACCTTAAACGCATCTTCGTCAGTACGGTCATCCCCGATATACACCGG
CACAACATCATCCGAATTTTCATATCCTATTTTAATTAGTCGATAAATGATATGAGAACT
TGAGATCTTTAAATTCTCATATCGAAATGAAAAATAAATAAGATTTATGAGGGGCTCTCT
CACCTAATGATTTTAGTAAAAAATTGAGTGCCTGGCCCTTGTCCCATTTGATTGTGGGGC
GGATTTCAGTA
Primers used for PCR NE71 - 8474, show primer details
   
Sequence Name 72-K104216-0022-596-G04C-NE71
GenBank Accession LN512719 [GenBank]
Sequencing primer gene specific primer, NE71: 5'-TAAAATCATTAGGTGAGAGAGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K104216-0022-596-G04C-NE71
GAGAATTAAAGATCTCAAGTTCTCATATCATTTATCGACTAATTAAAATAGGATATGAAA
ATTCGGATGATGTTGTGCCGGTGTATATCGGGGATGACCGTACTGACGAAGATGCGTTTA
AGGTATAGTGACACTTAACTTATACGTATTCGTAACATCAGTTAATTAAGTCTTTTTACA
TTTTGAACAATTTGATTCTATTACTGACGTAATAAATGAACTGTGTAATTCGATAGGTTT
TACGTGAAAGGGGACAAGGTTTTGGGATTCTTGTCTCAAAAGTACCAAAGGACACCAATG
CCTCTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGC
   
Sequence Name 32-K104217-0022-596-G04C-OriL
GenBank Accession LN500566 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K104217-0022-596-G04C-OriL
TTTGGTACTTTTGAGACAAGAATCCCAAAACCTTGTCCCCTTTCACGTAAAACCTATCGA
ATTACACAGTTCATTTATTACGTCAGTAATAGAATCAAATTGTTCAAAATGTAAAAAGAC
TTAATTAACTGATGTTACGAATACGTATAAGTTAAGTGTCACTATACCTTAAACGCATCT
TCGTCAGTACGGTCATCCCCGATATACACCGGCACAACATCATCCGAATTTTCATATCCT
ATTTTAATTAGTCGATAAATGATATGAGAACTTGAGATCTTTAAATTCTCATATCGAAAT
GAAAAATAAATAAGATTTATGAGGGGCTCTCTCAC

Last Updated on 06.12.2018 16:11