Sequences from confirmation amplicon


Confirmation sequences for line 596G12 and hit At5g25280 are shown.
Line ID 596G12
Locus At5g25280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8774193
Primers used for PCR BC48 - 8474, show primer details
   
Sequence Name 48-K112877-0022-596-G12C-8409
GenBank Accession LN506548 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112877-0022-596-G12C-8409
ATTTCATTGAATATATCCATCGCTGTTTCTTCCACAAACCTAGCAATCATCACATAATTC
CGGATTCGAGGAGAAGATGTATGTGCTCTCCGACAACGCATCCTGGTTCATTCCGGTGTA
GTCTACACAAGAACGTCGCGAATCCACATGGTCAAGGTACAGCTTCGTATACAACGAATG
GATTGAATATGCGTCGATCTGCGATGACGAATTCTCTTGTGAGAATTGGTGGTGTTGAAG
GTGAATGGGTGCGAAGAGCTTTGACTACTTTGATTAGACCATCTTCACATCAACTAAAGA
GAAGATCTGCTTATGAGCCTCGTCGTAGTAGACTCGCATCTATGTCGAAAGCAGAAGATC
TTTG
   
Sequence Name 85-K112878-0022-596-G12C-BC48
GenBank Accession LN514566 [GenBank]
Sequencing primer gene specific primer, BC48: 5'-CAAAGATCTTCTGCTTTCGACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K112878-0022-596-G12C-BC48
TAGCAGATCTTCTCTTTAGTTGATGTGAAGATGGTCTAATCAAAGTAGTCAAAGCTCTTC
GCACCCATTCACCTTCAACACCACCAATTCTCACAAGAGAATTCGTCATCGCAGATCGAC
GCATATTCAATCCATTCGTTGTATACGAAGCTGTACCTTGACCATGTGGATTCGCGACGT
TCTTGTGTAGACTACACCGGAATGAACCAGGATGCGTTGTCGGAGAGCACATACATCTTC
TCCTCGAATCCGGAATCTTGTGATGATTGCTAGGTTTGTTGGAAGAAACAGCGATGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATA

Last Updated on 06.12.2018 16:11