Sequences from confirmation amplicon


Confirmation sequences for line 596H04 and hit At2g36250 are shown.
Line ID 596H04
Locus At2g36250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15199892
Primers used for PCR IH22 - 8474, show primer details
   
Sequence Name 65-K104216-0022-596-H04C-IH22
GenBank Accession LN511392 [GenBank]
Sequencing primer gene specific primer, IH22: 5'-ACCCCTAGTCAACTCCTTACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K104216-0022-596-H04C-IH22
GCAACAGGAGACATTCTCATAGCCTGGATATCAGTGTTGACAATCCAGAACTCCACACCT
GACATTTCACTCTCTATCATACGATTCACAGCATTTGATCCACCACCTCCCACACCAATA
ACCTTAATCCTCGCCTCATTGTAGTTACTCGGAGCAGATGGCTCTTCAAAATCCTCGACA
ACAGGTCCAGAAGACGTTTCCTTTCTTGGATTGACTATTGTACTAGTCCCTTCACCTCTA
AGCATAGATATTTCCGGGTGAAGGTTCAAGAAAGGATCTTGAGCTTGGCTTTGGTAATGC
CGTGGAGAGTTTCTGATTGGAGAAGATTCAGATTTCTGAGCGGCAACAACAACTCGGTTT
TTCTTACTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 25-K104217-0022-596-H04C-OriL
GenBank Accession LN497420 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104217-0022-596-H04C-OriL
AAACCGAGTTGTTGTTGCCGCTCAGAAATCTGAATCTTCTCCAATCAGAAACTCTCCACG
GCATTACCAAAGCCAAGCTCAAGATCCTTTCTTGAACCTTCACCCGGAAATATCTATGCT
TAGAGGTGAAGGGACTAGTACAATAGTCAATCCAAGAAAGGAAACGTCTTCTGGACCTGT
TGTCGAGGATTTTGAAGAGCCATCTGCTCCGAGTAACTACAATGAGGCGAGGATTAAGGT
TATTGGTGTGGGAGGTGGTGGATCAAATGCTGTGAATCGTATGATAGAGAGTGAAATGTC
AGGTGTGGAGTTCTGGATTGTCAACACTGATATCCAGGCTATGAGAATGTCTCCTGTTTT
GCCTGATAATAGGTTACAAATTGGTAAG
Primers used for PCR GC58 - 8474, show primer details
   
Sequence Name 66-K104216-0022-596-H04C-GC58
GenBank Accession LN511601 [GenBank]
Sequencing primer gene specific primer, GC58: 5'-CTAGTCAGAAAACACCAAAACCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K104216-0022-596-H04C-GC58
GCTCCTTGTTCAATTCACACTTTTTCTCTCCCAACTCTTCTTCCAGGTTTCCTCTGCTAC
TCTCAGGTTTCTCTTCTCCACTTCCCTCCAGCTTTATCCTTTTGATTTCGTCCTCCATCT
TACTGTATTTACCCAAAAATCTAAGCTTTTCTCAGTGTTCTTCCATTTCTCTACTTTGTT
TTCATGTAATGCTGCAAACTTCGCAATACCCATCTTCAATTTCTTCAATTCGTAATGGGA
AAAAGCATTTTAGTTTAATTTGGGTTTTGTGGTTCCTCACTTGTTTCATTCTTCTAAGTC
TTAAACCATCCACTGATTCTCAATTTCTGAGGATTTGATATCTATTGCTGGTTAACAATG
GAGCATAGATGCGACTGGGTTTTGTTTATGTTAACTTTATGAATGTAAATCACACTGCTT
TGTGGCTACTTTGTGGATTTCGTGTTTTACTTTGTGGCTGAATCATATAACTCTAAATTG
AGAGCTTTGGGTTTGACCATTTAGCTTTTTATGAACAGGTCTTGTTAGGGAAGTAATCAG
TGGAATTGGATTTAGTCTTTTGGTTCTGAATTTTGTAAAGTCCTGCAGTGATTGGTCTCA
TTATGGCAACTTACGTTTCACCGTGTTTTACTCCTTCGGATTCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 26-K104217-0022-596-H04C-8409
GenBank Accession LN497902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104217-0022-596-H04C-840g
ATTGAATATATCCTGAATCCGAAGGAGTAAAACACGGTGAAACGTAAGTTGCCATAATGA
GACCAATCACTGCAGGACTTTACAAAATTCAGAACCAAAAGACTAAATCCAATTCCACTG
ATTACTTCCCTAACAAGACCTGTTCATAAAAAGCTAAATGGTCAAACCCAAAGCTCTCAA
TTTAGAGTTATATGATTCAGCCACAAAGTAAAACACGAAATCCACAAAGTAGCCACAAAG
CAGTGTGATTTACATTCATAAAGTTAACATAAACAAAACCCAGTCGCATCTATGCTCCAT
TGTTAACCAGCAATAGATATCAAATCCTCAGAAATTGAGAATCAGTGGATGGTTTAAGAC
TTAGAAGAATGAAACAAGTGAGGAACCACAAAACCCAAATTAAACTAAAATGCTTTTTCC
CATTACGAATTGAAGAAATTGAAGATGGGTATTGCGAAGTTTGCAGCATTACATGAAAAC
AAAGTAGAGAAATGGAAGAACACTGAGAAAAGCTTAGATTTTTGGGTAAATACAGTAAGA
TGGAGGACGAAATCAAAAGGATAAAGCTGGAGGGAAGTGGAGAAGAGAAACCTGAGAGTA
GCAGAGGAAACCTGGAAGAAGAGTTGGGAGAGAAAAAGTGTGAATTGAACAAGGAGCTCA
AGGTCTGGAGGAAACCCACGAAACG

Last Updated on 06.12.2018 16:11