Genotyping and primer details


Genotyping and primer details for 596H04 - At2g36250
Line ID 596H04
Locus At2g36250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15199892 of line 596H04 Chr2:15199892
   
Insertion allele amplicon 8474 - GC58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GC58: 5'-CTAGTCAGAAAACACCAAAACCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15200587
   
Insertion allele amplicon 8474 - IH22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IH22: 5'-ACCCCTAGTCAACTCCTTACCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15199402
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11