Sequences from confirmation amplicon


Confirmation sequences for line 597E06 and hit At4g34180 are shown.
Line ID 597E06
Locus At4g34180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16370556
Primers used for PCR 63ns - 8474, show primer details
   
Sequence Name 60-K114716-0022-597-E06C-63ns
GenBank Accession LN510329 [GenBank]
Sequencing primer gene specific primer, 63ns: 5'-ATATAGGTCCTGCTTTGTTGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K114716-0022-597-E06C-63ns
TTGATTTGTAACGAAAATTAAAGTTAGTATCTTTTGTCTAATGATTTGTGTTTGGGTTGG
ATTATTATTTATTAGCTGAGGTAATGGAATCACTTCATATACAAAGAGGAGTTCGTCGTG
TGCTCTTTAGAACATCCAACACCGACNAGTAAGAGTCTCAGTATTGAGAGTGTGTGTTAT
TACCTTAATCTTGGTAAAAACAACTAGAGAGCATAAGCTTACTCACTCTGTGTTTGATAT
ATATATTAGGCGGCTTATGTTTAAGAAAGAGTTTGATTCAAGCTTTGCTGGGTTCATGAC
CGATGGGGCTAAATGGTTGGTTGAGAATACAGACATCAATGAGTATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K114715-0022-597-E06C-8409
GenBank Accession LN491687 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114715-0022-597-E06C-8409
ATTTACATTGAATAATACTCATTGATGTCTGTATTCTCAACCAACCATTTAGCCCCATCG
GTCATGAACCCAGCAAAGCTTGAATCAAACTCTTTCTTAAACATAAGCCGCCTAATATAT
ATATCAAACACAGAGTGAGTAAGCTTATGCTCTCTAGTTGTTTTTACCAAGATTAAGGTA
ATAACACACACTCTCAATACTGAGACTCTTACTTGTCGGTGTTGGATGTTCTAAAGAGCA
CACGACGAACTCCTCTTTGTATATGAAGTGATTCCATTACCTCAGCTAATAAATAATAAT
CCAACCCAAACACAAATCATTAGACAAAAGATACTAACTTTAAATTTTCGTTACAAATTC
AACAACTCACCAGTAATGTTCTTATCTCTCGGAACATCAACCAACAAAGCAGAACCTATA

Last Updated on 06.12.2018 16:11