Sequences from confirmation amplicon


Confirmation sequences for line 599E02 and hit At1g66800 are shown.
Line ID 599E02
Locus At1g66800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24925984
Primers used for PCR 21vi - 8474, show primer details
   
Sequence Name 20-K117806-0022-599-E02C-21vi
GenBank Accession LN495398 [GenBank]
Sequencing primer gene specific primer, 21vi: 5'-ATGGATATTCAAAAACTTTGGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117806-0022-599-E02C-21vi
CGGGATCGATTTGGTTGTAATGAATCCAGGAAACGTGATCGGACCAGTTTTGCAGCCAAC
TCTTAATTACTCTGTAGAAGTGATTGTGGATCTGATAAATGGTAAAAACCCTTCCAATAG
TTTTTACTATAGGTTCATGGACGTGAGAGATGTTTCTCTTGCTCATATCAAAGCGTTCGA
GGTCCCTTCTGCTAGTGGCAGATACATACTTGCTGATCCAGATGTAACAATGAAAGACAT
TCAAAAGCTTTTACATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAAGATTATTAT
   
Sequence Name 20-K117784-0022-599-E02C-8409
GenBank Accession LN495397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117784-0022-599-E02C-8409
CCATTTACATTGAATATATCATGTAAAAGCTTTTGAATGTCTTTCATTGTTACATCTGGA
TCAGCAAGTATGTATCTGCCACTAGCAGAAGGGACCTCGAACGCTTTGATATGAGCAAGA
GAAACATCTCTCACGTCCATGAACCTATAGTAAAAACTATTGGAAGGGTTTTTACCATTT
ATCAGATCCACAATCACTTCTACAGAGTAATTAAGAGTTGGCTGCAAAACTGGTCCGATC
ACGTTTCCTGGATTCATTACAACCAAATCGATCCCGTTTTCTTTAGCAAACCGCCACGCT
GTTTCCTCAGCCAAAGTTTTTGAATATCCAT

Last Updated on 06.12.2018 16:11