Sequences from confirmation amplicon


Confirmation sequences for line 599G06 and hit At3g28340 are shown.
Line ID 599G06
Locus At3g28340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10590002
Primers used for PCR BC09 - 8474, show primer details
   
Sequence Name 03-K103979-0022-599-G06C-BC09
GenBank Accession LN487461 [GenBank]
Sequencing primer gene specific primer, BC09: 5'-GATGTCTGGTTCAAGATTAGCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103979-0022-599-G06C-BC09
ATAATCTCACATCTTTCTTCACCGTTGAATCGATTCGACTATTCCCTGATTCATTCGACG
ATGCATCTTCAGATTTAATGGAAGCTCCAGCATATCAAAACGGTCTTGATTGCTCTGTTT
TAGCCAAAAACAGACTCTTGTTAGCTTGTGATCCATCAGCTGTTCATATAGCTATGACTC
TAGATCCAGCTTACTTGCGTGGCACGGTATCTGCAGTACATTCCATCCTCAAACACACTT
CTTGCCCTGAAAACATCTTCTTCCACTTCATTGCTTCGGGTACAAGTCAGGGTTCCCTCG
CCAAGACCCTATCCTCTGTTTTTCCTTCTTTGAGTTTCAAAGTCTATACCTTTGAAGAAA
CCACGGTCAAGAATCTAATCTCTTCTTCTATAAGACAAGCTCTTGATAGTCCTTTGAATT
ACGCAAGAAGCTACTTATCCGAGTGATATATTCAGAATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K103980-0022-599-G06C-8409
GenBank Accession LN494733 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103980-0022-599-G06C-8409
GGAATGAGCCTTTACATTGAATATATTCTGAATATATCACTCGGATAAGTAGCTTCTTGC
GTAATTCAAAGGACTATCAAGAGCTTGTCTTATAGAAGAAGAGATTAGATTCTTGACCGT
GGTTTCTTCAAAGGTATAGACTTTGAAACTCAAAGAAGGAAAAACAGAGGATAGGGTCTT
GGCGAGGGAACCCTGACTTGTACCCGAAGCAATGAAGTGGAAGAAGATGTTTTCAGGGCA
AGAAGTGTGTTTGAGGATGGAATGTACTGCAGATACCGTGCCACGCAAGTAAGCTGGATC
TAGAGTCATAGCTATATGAACAGCTGATGGATCACAAGCTAACAAGAGTCTGTTTTTGGC
TAAAACAGAGCAATCAAGACCGTTTTGATATGCTGGAGCTTCCATTAAATCTGAAGATGC
ATCGTCGAATGAATCAGGGAATAGTCGAATCGATTCAACGGTGAAGAAAGATGTGGAGAT
TATTGAGAAGATTATTATTAGTCTAGAGGCTAATCTT

Last Updated on 06.12.2018 16:11