Sequences from confirmation amplicon


Confirmation sequences for line 600G05 and hit At2g43570 are shown.
Line ID 600G05
Locus At2g43570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18076864
Primers used for PCR NG10 - 8474, show primer details
   
Sequence Name 20-K102364-0022-600-G05C-8409
GenBank Accession LN495157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102364-0022-600-G05C-8409
GCCATTTACAATTGAATATGTCCACCACTATAGGTTTCGTTTTTGTGACCATATGGTTAT
ACCTTGTTGCAGTCATGTGTTACATTGAGGAAATTGATGGACCAGCCAAGGCCGCATCCG
GAGAGTACTGCGACACAGAGAAACCAGAGTTCCCATGTGCACAAGGAAAGGGCTACTATG
GCCGTGGTGCAATCCAGCTCTCTTGGAACTACAACTACGGTCTCTGTGGCAAAGCCTTGG
ACGAGAACTTATTGGCTTCACCAGAGAAAGTTGCTCAAGACCAGGTTCTTGCCTTCAAGA
CCGCTTTCTGGTTCTGGACCACTAATGTTCGCACGAGTTTTAAATCGGGCTTTGGCGCAA
CAATCAGGGCAGTGAATAGTAGGGAGTGTAGCGGAGGAGATTCTACAGCCAAAGCCGCCA
ACAGGATTAAGTATTTCCAAGACTATTGTGGCAAGCTTGGAGTTGCACCTGGAGATAACC
TCACTTG
   
Sequence Name 20-K102365-0022-600-G05C-NG10
GenBank Accession LN495158 [GenBank]
Sequencing primer gene specific primer, NG10: 5'-GCAAGGAAACTTTAACAAGTGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102365-0022-600-G05C-NG10
TAGTCTTGGGAAACTTAATCCTGTTGGCGGCTTTGGCTGTAGAATCTCCTCCGCTACACT
CCCTACTATTCACTGCCCTGATTGTTGCGCCAAAGCCCGATTTAAAACTCGTGCGAACAT
TAGTGGTCCAGAACCAGAAAGCGGTCTTGAAGGCAAGAACCTGGTCTTGAGCAACTTTCT
CTGGTGAAGCCAATAAGTTCTCGTCCAAGGCTTTGCCACAGAGACCGTAGTTGTAGTTCC
AAGAGAGCTGGATTGCACCACGGCCATAGTAGCCCTTTCCTTGTGCACATGGGAACTCTG
GTTTCTCTGTGTCGCAGTACTCTCCGGATGCGGCCTTGGCTGGTCCATCAATTTCCTCAA
TGTAACACATGACTGCAACAAGGTATAACCATATGGTCACAAAAACGAAACCTATAGTGG
TGGACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
AATGATGGTCAATATA

Last Updated on 06.12.2018 16:11