Sequences from confirmation amplicon


Confirmation sequences for line 600H03 and hit At5g46210 are shown.
Line ID 600H03
Locus At5g46210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18732636
Primers used for PCR BD78 - 8474, show primer details
   
Sequence Name 21-K028348-022-600-H03C-8409
GenBank Accession LN495530 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K028348-022-600-H03C-8409
ATTGGTAATCATTGCTCTTTTCTTTTTGTCTCAGGCTGTTGTGTTAATGTTATTTAACGA
TGCAATGAAACCTTAGCTTTGAAGACATTAAAGATTCCACCAGCATAGAGGACAAGGAGC
TGAGGAGGACTTTGCAGTCGCTTGCTTGTGGGAAAGTCCGTGTCCTGCAGAAGGTTTGGC
CAAAATCTGGACTCTGTTAACTCTCTTTTCAGCTAATGCAGAAACTGCCGAGATTATGGC
TTTGCCTTTTTCCATAAGGATAAGCCTTATTGTGTAAATATTTTTGCAGAATCCTAAGGG
AAGAGATGTAGAAGACGGTGATGAATTTGAATTTAATGATGAGTTTGCAGCTCCGCTGTA
TCGCATTAAGGAACCGTTGG
   
Sequence Name 21-K028348-022-600-H03C-BD78
GenBank Accession LN495531 [GenBank]
Sequencing primer gene specific primer, BD78: 5'-AACCAACGTACCTTAATGCGATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K028348-022-600-H03C-BD78
CCTTAAGGAATTCTGCAAAAATATTTACACAATAAGGCTTATCCTTATGGAAAAAGGCAA
AGCCATAATCTCGGCAGTTTCTGCATTAGCTGAAAAGAGAGTTAACAGAGTCCAGATTTT
GGCCAAACCTTCTGCAGGACACGGACTTTCCCACAAGCAAGCGACTGCAAAGTCCTCCTC
AGCTCCTTGTCCTCTATGCTGGTGGAATCTTTAATGTCTTCAAAGCTAAGTTTCATTGCA
TCGTTAAATAACATTAACACAACAGCCTGAGACAAAAAGAAAAGAGCAATTGATTACCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATT

Last Updated on 06.12.2018 16:11