Sequences from confirmation amplicon


Confirmation sequences for line 602B10 and hit At5g60980 are shown.
Line ID 602B10
Locus At5g60980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24543339
Primers used for PCR 03jq - 8474, show primer details
   
Sequence Name 74-K113079-0022-602-B10C-8409
GenBank Accession LN513106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113079-0022-602-B10C-8409
AATTAAACTAAACTAAAGTTGGTACGGTTCCTTCTCTTCTCTCTCTCTCTTTCTCCGTCT
GTCGCTGTCTCTCTCGTCCTCTCCTCTGTTTCGTAGCTTCTGCAGCAGATCTCTCTTTGT
CCTTCTCTCTCCCTCAGATCACTGTAAGTACACGACACTTCACTTCATTTCCTAATTCGA
TCTGTTTCTTTTACTCTTTCTCGATTTGCTTCCAATCTTGATCTCTTTTCTCTTTCTATA
TCGAGCTTGATCTGACTCATTCTTTATTATATACTTCTCTTCTCGATTCCTTTTTTGTGA
AAAAAACATCTTTAAAATTTCATTTTTGTTAGTTGCTTCGTG
   
Sequence Name 68-K113078-0022-602-B10C-03jq
GenBank Accession LN512049 [GenBank]
Sequencing primer gene specific primer, 03jq: 5'-CACGAAGCAACTAACAAAAATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113078-0022-602-B10C-03jq
GATCGAGAAGAGAAGTTATAATAAAGAATGAGTCAGATCAAGCTCGATATAGAACGAGAA
AAGAGATCAAGATTGGAAGCAAATCGAGAAAGAGTAAAAGAAACAGATCGAATTAGGAAA
TGAAGTGAAGTGTCGTGTACTTACAGTGATCTGAGGGAGAGAGAAGGACAAAGAGAGATC
TGCTGCAGAAGCTACGAAACAGAGGAGAGGACGAGAGAGACAGCGACAGACGGAGAAAGA
GAGAGAGAGAAGAGAAGGAACCGTACCAACTTTAGTTTTAGTTTAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTAT

Last Updated on 06.12.2018 16:11