Sequences from confirmation amplicon


Confirmation sequences for line 604B09 and hit At2g16220 are shown.
Line ID 604B09
Locus At2g16220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7031125
Primers used for PCR 27ya - 8474, show primer details
   
Sequence Name 82-K119624-0022-604-B09C-27ya
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 27ya: 5'-GAGAAGGAAAGTCCTTAGGTGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K119624-0022-604-B09C-27ya
AGGAGACCCTACTTTATTCTATAAGATTGCTAGGTTTTTCTAGGGCTTTTGTTAATTAGG
TTATCTAGGTGTCTTAATATTTAAGTCTTAACAAATCTCCTTCTTCTTCGTTCGATCGGC
CATCATGAATTCACATTTCTTAACGAATGATCTCATCCTGGAGGTTTTGTCAAGATTGCC
TTTAAAGTCAGTCGCGAGGTTTCATTGCGTGTCAAAGCGGTGGGCATCAATGTTTGGTAG
TCCATATTTCAAAGAGTTGTTCCTGACCAGATCCTCTGCTAAGCCACGCCTCTTATTTGC
CATCGTACAAAACGGTGTGTGGAGATTCTTTTCATCTCCTCGGTTAGAGAAGTCGTCGTC
GACTCTTGTAGCAACCGCAATGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGACCGCACGGTTATT
   
Sequence Name 58-K119623-0022-604-B09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K119623-0022-604-B09C-8409
CATTTCTCATTGCGGTTGCTACAGAGTCGACGACGACTTCTCTAACCGAGGAGATGAAAA
GAATCTCCACACACCNGTTTTGTACGATGGCAAATAAGAGGCGTGGCTTAGCAGAGGATC
TGGTCAGGAACAACTCTTTGAAATATGGACTACCAAACATTGATGCCCACCGCTTTGACA
CGCAATGAAACCTCGCGACTGACTTTAAAGGCAATCTTGACAAAACCTCCAGGATGAGAT
CATTCGTTAAGAAATGTGAATTCATGATGGCCGATCGAACGAAGAAGAAGGAGATTTGTT
AAGACTTAAATATTAAGACACCTAGATAACCTAATTAACAAAAGCCCTAGAAAAACCTAG
CAATCTTATAGAATAAAGTAGGGTTCTCCTTATAACTCACTTCACCAGAAGTTGATACGT
GTCACCTAAGGACTTTCCTTCT

Last Updated on 06.12.2018 16:11