Sequences from confirmation amplicon


Confirmation sequences for line 604E11 and hit At3g05420 are shown.
Line ID 604E11
Locus At3g05420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1566319
Primers used for PCR 18vc - 8474, show primer details
   
Sequence Name 60-K117671-0022-604-E11C-18vc
GenBank Accession LN510357 [GenBank]
Sequencing primer gene specific primer, 18vc: 5'-CTGTTAATAATGCCACAACCAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K117671-0022-604-E11C-18vc
CAGAGGCAGGGTACGGGAAATTGTCATGGACAATGTTAACCCTGGATCAAAGGTATCTTC
TTGTCAAATTTCCCTCCTTAATATATTTTGCATAGCACCACCTTTTGATTTGTTTGAGAG
AATTGAAGCATCTTATGCATATATGCTGTGGTAGTGATTGTGTCGACACAGAATATAGTT
ACTCCAGTCCGGATCATCACCTTCTCGCACTCTTATTATGTTTTGGGTGATTAATCCCCA
GGTTGAAGGAAACAGCGAACGCATTATTGCGACTATTAAATCTGAGAAGGAAGAGCTGGA
GGCATCACTGAACAAAGAGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 12-K117670-0022-604-E11C-8409
GenBank Accession LN491770 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117670-0022-604-E11C-8409
ATTTCATTGAATAATCCTCTCTTTGTTCAGTGATGCCTCCAGCTCTTCCTTCTCAGATTT
AATAGTCGCAATAATGCGTTCGCTGTTTCCTTCAACCTGGGGATTAATCACCCAAAACAT
AATAAGAGTGCGAGAAGGTGATGATCCGGACTGGAGTAACTATATTCTGTGTCGACACAA
TCACTACCACAGCATATATGCATAAGATGCTTCAATTCTCTCAAACAAATCAAAAGGTGG
TGCTATGCAAAATATATTAAGGAGGGAAATTTGACAAGAAGATACCTTTGATCCAGGGTT
AACATTGTCCATGACAATTTCCCGTACCCTGCCTTCTTGGCTCACCTCAACCTCAGACTC
AATGTCTCTGGTTGTGGCATTATTAACA

Last Updated on 06.12.2018 16:11