Sequences from confirmation amplicon


Confirmation sequences for line 606G03 and hit At3g60000 are shown.
Line ID 606G03
Locus At3g60000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22160944
Primers used for PCR 34vf - 8474, show primer details
   
Sequence Name 30-K117753-0022-606-G03C-34vf
GenBank Accession LN499897 [GenBank]
Sequencing primer gene specific primer, 34vf: 5'-GAGAGTGTAAAAGGCGAGTCTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117753-0022-606-G03C-34vf
TTTAACTGACGTCTAAGTCTCTGAGCAGAAACAGAGCGTTGAATCTGGTGGTGTTGAAGG
TGGTGCTGCTTTGGAGAATTACAAGTCAAAGAGTGGAGATCTCCGGCGGAGGAAGAGGAT
GAAGTAACCGGAGACATGAATCTGGAACTTACTTCTCTAACTCTTGGCCGCCGCGTTGAA
GGCGGAGGAGCCGGTGTTGATGGCTGAATTTGCTTAGGTCCGCTGGTTTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K117752-0022-606-G03C-8409
GenBank Accession LN492695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117752-0022-606-G03C-8409
AAACAGCGGACCAAGCAAATTCAGCCATCAACACCGGCTCCTCCGCCTTCAACGCGGCGG
CCAAGAGTTAGAGAAGTAAGTTCCAGATTCATGTCTCCGGTTACTTCATCCTCTTCCTCC
GCCGGAGATCTCCACTCTTTGACTTGTAATTCTCCAAAGCAGCACCACCTTCAACACCAC
CAGATTCAACGCTCTGTTTCTGCTCAGAGACTTAGACGTCAGTTAAAAATGGCAGACGGA
GATGAGAATCGCTCATCAGAGACGGCGGCGCGTAGCCTAGACTCGCCTTTAACACTCTC

Last Updated on 06.12.2018 16:11