Genotyping and primer details


Genotyping and primer details for 606G03 - At3g60000
Line ID 606G03
Locus At3g60000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22160944 of line 606G03 Chr3:22160944
   
Insertion allele amplicon 8474 - 34vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34vf: 5'-GAGAGTGTAAAAGGCGAGTCTAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22161240
   
wt amplicon (over InsPos) 81vo - 34vf
wt amplicon start Chr3:22160740
wt amplicon end Chr3:22161240
wt amplicon size 501 bp
Locus specific primer 34vf: 5'-GAGAGTGTAAAAGGCGAGTCTAGG-3'
2nd locus specific primer 81vo: 5'-ATTAATGTGGCGTTACAGAGACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11