Sequences from confirmation amplicon


Confirmation sequences for line 607C02 and hit At3g60900 are shown.
Line ID 607C02
Locus At3g60900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22500382
Primers used for PCR 20lu - 8474, show primer details
   
Sequence Name 44-K114180-0022-607-C02C-20lu
GenBank Accession LN505262 [GenBank]
Sequencing primer gene specific primer, 20lu: 5'-GCTTTCACATTATCTCTCCTCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K114180-0022-607-C02C-20lu
TCAATCCTCTCTGATACGCCGGAATATTCCTCATTCAACAACTACCTCTTTAAACAAAAC
TCGCCGACGAAATCAACAGCCGCACTACAATCACCGTCCTTGTTCTCAACAATGGCGCAA
TGTCTTCCCTCGCCGGAAAACACCCACTCTCCGTCGTCAAAAATGCTCTCAGCCTCCTCG
TCCTCCTCGACTACTACGATCCTTTAAAACTCCATCAGCTCTCTAAAGGCACAACTCTCA
CCACCACGCTTTACCAAACCACCGGACACGCTCTCGGGAACCTAGGATTCGTTAACGTCA
CCGATCTCAAAGGAGGCAAAGTCGGCTTTGGCTCCGCCGCTCCTGGCTCCAAGCTCGATT
CCTCTTACACCATCTCCGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACGATTATTA
   
Sequence Name 08-K114177-0022-607-C02C-8409
GenBank Accession LN489819 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114177-0022-607-C02C-8409
TCGGAGATGGTGTAGAGGAATCGAGCTTGGAGCCAGGAGCGGCGGAGTCAAAGCCGACTT
TGCCTCCTTTGAGATCGGTGACGTTAACGAATCCTAGGTTCCCGAGAGCGTGTCCGGTGG
TTTGGTAAAGCGTGGTGGTGAGAGTTGTGCCTTTAGAGAGCTGATGGAGTTTTAAAGGAT
CGTAGTAGTCGAGGAGGACGAGGAGGCTGAGAGCATTTTTGACGACGGAGAGTGGGTGTT
TTCCGGCGAGGGAAGACATTGCGCCATTGTTGAGAACAAGGACGGTGATTGTAGTGCGGC
TGTTGATTTCGTCGGCGAGTTTTGTTTGAGAAAGGTAGTTGTTGAATGAGGAATATTCCG
GCGTATCAGAGAGGATTTGAGTAATGTTATGGCCGGAGACGGTAGAAGCTACGGTGAGGA
GAGATAATGTGAAAG

Last Updated on 06.12.2018 16:11