Sequences from confirmation amplicon


Confirmation sequences for line 608D10 and hit At5g25420 are shown.
Line ID 608D10
Locus At5g25420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8839348
Primers used for PCR NJ55 - 8474, show primer details
   
Sequence Name 69-K036619-0022-608-D10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K036619-0022-608-D10C-8409
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGATATCTTTATTTCGACGTGTCTACATTC
ACGT
   
Sequence Name 70-K036619-0022-608-D10C-NJ55
GenBank Accession LN512328 [GenBank]
Sequencing primer gene specific primer, NJ55: 5'-CTTTCGACATCACTGATTCTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K036619-0022-608-D10C-NJ55
TCTCTTTGAGTTAACTTTATTCTTTACATGAAACTGATGGAAAATAAAATTTAGCATACT
ATGGTTAAATATGAAGAGTGATAAGTTTTATGTACTATTGTAGTCGACTGGTTTGTTCTA
TGCATCGGCAAGATATGGAAGTGCGACGCCAATTCCACCATCAGTTGTTAGTCGTGGTAC
TGGTTGGCTGGTTAGTCTACAAACAATTTAATTTTCACTTTAAAACATATTTTTTTGACC
ATTTATTAACTATTTATTTCATCAATTGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11