Sequences from confirmation amplicon


Confirmation sequences for line 609A07 and hit At5g45670 are shown.
Line ID 609A07
Locus At5g45670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18528702
Primers used for PCR HM11 - 8474, show primer details
   
Sequence Name 11-K028919-022-609-A07C-8409
GenBank Accession LN490977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K028919-022-609-A07C-8409
CACCAAATATGAAGTAACATGGTGCAATGGGATCACTCTTAGCAATGTTAATCATGGTCA
CAGCAACCATAATCATCATGATCATCAAACTCATTCTCGCCATCTTTTTCTAGGAGCAAG
ATAGTGAGTGAATGGGAGAAGAAAGAGAGAGATGTTTTGTGAATGTGAAGAAACGAAAAA
TGTGTTTATATAATATAGTTTTGAAGAATATGGAGAGAATGAGAAACTGTTATTTTATAA
AGTTTTTTAACGGTTGAAGAAGCATTAAATGTTCACGCGGCAATGATGTTTGGATGTTAA
ATGTAAAAGTAAATGTGATGTGTGTTTATATATGTGAAATAATTGAGTATTTTTGGTGGA
ACTATGAATTGATCCATCAGAGACATATAACTAAAGATGGTGGAGAGTTATTACTTCTGT
CAATGTTGTTTATTTTCTTTACTCTGAATCCCCCCAACTTGTATGCATTGTGACGGTTCG
AT
   
Sequence Name 12-K028919-022-609-A07C-HM11
GenBank Accession LN491437 [GenBank]
Sequencing primer gene specific primer, HM11: 5'-AAAACATTAAAATCGAACCGTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028919-022-609-A07C-HM11
GGATCAATTCATANGTTCCACCAAAAATACTCAATTATTTCACATATATAAACACACATC
ACATTTACTTTTACATTTAACATCCAAACATCATTGCCGCGTGAACATTTAATGCTTCTT
CAACCGTTAAAAAACTTTATAAAATAACAGTTTCTCATTCTCTCCATATTCTTCAAAACT
ATATTATATAAACACATTTTTCGTTTCTTCACATTCACAAAACATCTCTCTCTTTCTTCT
CCCATTCACTCACTATCTTGCTCCTAGAAAAAGATGGCGAGAATGAGTTTGATGATCATG
ATGATTATGGTTGCTGTGACCATGATTAACATTGCTAAGAGTGATCCCATTGCACCATGT
TACTTCATATTTGGTGACTCTTTGGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11