Sequences from confirmation amplicon


Confirmation sequences for line 609A08 and hit At1g15180 are shown.
Line ID 609A08
Locus At1g15180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5226318
Primers used for PCR CH71 - 8474, show primer details
   
Sequence Name 36-K107000-0022-609-A08C-8409
GenBank Accession LN502241 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K107000-0022-609-A08C-8409
ATTTACAATTGAATATATCCTGAATCCAGCCGGTGCCGTTCTGCAAACGCTTCTGCTTAC
TCTTGTCACGGGCTGCACAAACTGGGAAAGCCAGGTCCTACTTCTTTTTCGTTCTTTTCT
TCTTCATGACTAGTTTCTCTTGTGAAATAAACATACAGATCTTGAATTGGATGCATATTT
GAACTGTAGGCCGATAAAGCGAGAAATCGAATGGCTTTGGCCTATGGAACGTAAGGCGCT
GCAGAACAGCTAAAGATCATAGTGAGCATAGGTTCCTCCGGTTTTACCAGTTTTGTAGCA
GACGAATTGCTGTATGTTACTTTGTGGTTATTGGAAGGTTTG
   
Sequence Name 44-K107001-0022-609-A08C-CH71
GenBank Accession LN505198 [GenBank]
Sequencing primer gene specific primer, CH71: 5'-CAAACCTTCCAATAACCACAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107001-0022-609-A08C-CH71
CAAACTGGTAAACCGGAGGAACCTATGCTCACTATGATCTTTAGCTGTTCTGCAGCGCCT
TACGTTCCATAGGCCAAAGCCATTCGATTTCTCGCTTTATCGGCCTACAGTTCAAATATG
CATCCAATTCAAGATCTGTATGTTTATTTCACAAGAGAAACTAGTCATGAAGAAGAAAAG
AACGAAAAAGAAGTAGGACCTGGCTTTCCCAGTTTGTGCAGCCCGTGACAAGAGTAAGCA
GAAGCGTTTGCAGAACGGCACCGGCTTGGATTCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCATTATT

Last Updated on 06.12.2018 16:11