Sequences from confirmation amplicon


Confirmation sequences for line 609F04 and hit At1g01700 are shown.
Line ID 609F04
Locus At1g01700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:260095
Primers used for PCR 04kz - 8474, show primer details
   
Sequence Name 30-K113817-0022-609-F04C-04kz
GenBank Accession LN499804 [GenBank]
Sequencing primer gene specific primer, 04kz: 5'-TTTTCGAGTTTATCCCAAAGTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K113817-0022-609-F04C-04kz
GATTTGCTTATAGATATAAGTCCAAAACTGAAATAAACATCAATAGTTTAAAAATAGCTG
TTGCGGTTTTTTTGTCAGGTCATGGAGAGTAGACCAAGAGCAGATATCTACATCAATCTG
CCTGCACTCAGAAAACTAGATTCCATGCTTATGGTAGTTACTACACCAGAACAAGAAGCT
ATCATCAATAAGATTGACTCGGTTAATATTCCTCATAAAAGTCATTCTCTTTGTTTTCTG
CAGGAGGCATTGGATAGTTTTCAAAAGACAGAGTTTTGGTATGCAGAAGAAGGAAGCTTG
TCGATGAAGTCAACGCGTTCCGCAACTGGATCATTCAGGAAAGTGATTGTGCAGAGGAAA
GAAGAGAAATGGTGGTTACCGATTCCTCTGGTTCCATTACAAGGTTTATCAGAGAAGGCA
AGGAAACAACTTAAGAGCAAGCGAGAGAGCACTAATCAAATCCATAAAGCTGCTATGGCC
ATCAATAGCAGCATACTCGGTGAAATGGATATTCCTGATTCTTACATGGCAACTCTTCCA
AAGGTAACCACACTTAAGATGATTTTATGTCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K113816-0022-609-F04C-8409
GenBank Accession LN487997 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113816-0022-609-F04C-8409
GAATATATCCTGGACATAAAATCATCTTAAGTGTGGTTACCTTTGGAAGAGTTGCCATGT
AAGAATCAGGAATATCCATTTCACCGAGTATGCTGCTATTGATGGCCATAGCAGCTTTAT
GGATTTGATTAGTGCTCTCTCGCTTGCTCTTAAGTTGTTTCCTTGCCTTCTCTGATAAAC
CTTGTAATGGAACCAGAGGAATCGGTAACCACCATTTCTCTTCTTTCCTCTGCACAATCA
CTTTCCTGAATGATCCAGTTGCGGAACGCGTTGACTTCATCGACAAGCTTCCTTCTTCTG
CATACCAAAACTCTGTCTTTTGAAAACTATCCAATGCCTCCTGCAGAAAACAAAGAGAAT
GACTTTTATGAGGAATATTAACCGAGTCAATCTTATTGATGATAGCTTCTTGTTCTGGTG
TAGTAACTACCATAAGCATGGAATCTAGTTTTCTGAGTGCAGGCAGATTGATGTAGATAT
CTGCTCTTGGTCTACTCTCCATGACCTGACAAAAAAACCGCAACAGCTATNTTTAAACTA
TTGATGTTTATTTCAGTTTTTGGACTTATATCTATAAGCAAATCTTGTTTACCTCAACAG
TGGCTCCATTGCTTAGATTCTGTGACTTTGGG

Last Updated on 06.12.2018 16:11