Sequences from confirmation amplicon


Confirmation sequences for line 609F04 and hit At3g01680 are shown.
Line ID 609F04
Locus At3g01680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:252169
Primers used for PCR AM16 - 8474, show primer details
   
Sequence Name 55-K106193-0022-609-F04C-AM16
GenBank Accession LN508820 [GenBank]
Sequencing primer gene specific primer, AM16: 5'-AAGAAGAACAGAGAGAGAACCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K106193-0022-609-F04C-AM16
AGTTATTGCTGGTTCGTGTACACAATCTTTAGAGAGTGTGGTTTATAAGACCATCTATAA
CCACAAAGATTGATATCTAGGGGTAGGTGGAAACTGCGCAACCTAACCGAACCAAGATGG
TCAACATATTTATATTTAGACTGAATATCCTGAAACTGAACCAAAACACACCGAATTTAG
CCCGAACGAAACCTAATAACTTGGATGTTATTTTGGTGAGCCAAAAAAGTCTAAACAAAG
AATCACTTGAATTGTGGCAAACAAAAAGTGAATGTAGCTATACCTCGCAGGCAACACGAT
CGATAGCATAGGAGACACTATCAAGAACGCTCATCATGCTTGACTGCATCAGTTTATCCT
CAGTGGGCAACGGAAGCATCTGAAAAATCAAAGCAAGTACGTAAAGGAACATGATCATAC
GTACGTACTAAGAACGAAATTGATTGTGTGTAGGAATACTAACGGAGGCGTTGGTGTCTT
CAGAGTCAAGAGTGGCACGATCGAGAATATCTTCAACGAGAGAGAGAAGGCCACGCACTT
GGACTTCGCGAGCATCTGGGGAGTGAGTTTGTTGAATCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 71-K106194-0022-609-F04C-8409
GenBank Accession LN512563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K106194-0022-609-F04C-8409
CGGACTGAGCCATTTACATTGATTCAACAAACTCACTCCCCAGATGCTCGCGAAGTCCAA
GTGCGTGGCCTTCTCTCTCTCGTTGAAGATATTCTCGATCGTGCCACTCTTGACTCTGAA
GACACCAACGCCTCCGTTAGTATTCCTACACACAATCAATTTCGTTCTTAGTACGTACGT
ATGATCATGTTCCTTTACGTACTTGCTTTGATTTTTCAGATGCTTCCGTTGCCCACTGAG
GATAAACTGATGCAGTCAAGCATGATGAGCGTTCTTGATAGTGTCTCCTATGCTATCGAT
CGTGTTGCCTGCGAGGTATAGCTACATTCACTTTTTGTTTGCCACAATTCAAGTGATTCT
TTGTTTAGACTTTTTTGGCTCACCAAAATAACATCCAAGTTATTAGGTTTCGTTCGGGCT
AAATTCGGTGTGTTTTGGTTCAGTTTCAGGATATTCAGTCTAAATATAAATATGTTGACC
ATCTTGGTTCGGTTAGGTTGCGCAGTTTCCACCTACCCCTAGATATCAATCTTTGTGGTT
ATAGATGCTCTTATAAACCACACTCTCTAAAGATTGTTGTACACGTAACCAGCAATAACT
TGTTACAGAAGCAATACACTGTTTACGTGGTTCTCTCTCGGTTTCTTC

Last Updated on 06.12.2018 16:11