Sequences from confirmation amplicon


Confirmation sequences for line 613E06 and hit At1g08700 are shown.
Line ID 613E06
Locus At1g08700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2769954
Primers used for PCR 30et - 8474, show primer details
   
Sequence Name 59-K110926-0022-613-E06C-30et
GenBank Accession LN510029 [GenBank]
Sequencing primer gene specific primer, 30et: 5'-CCAGAGGAAATATGGAAGACAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K110926-0022-613-E06C-30et
GAGAATGTCTCCAGTTGAGTTGATGGGTTGGGGAGATAATAGGGAAGAAGCAAGAGGTTC
GGAGGAGTCGGATAACGTTGTGGACATTTCAAATAGAGGCATAAAACTTGGTCTTGGAGA
CTTTATTTTCTACAGTGTTCTTGTTGGTAGGGCAGCAATGTATGATCTGATGACTGTGTA
TGCCTGTTACCTTGCGATCATCTCCGGACTTGGGTGCACTCTTATTTTGTTATCTGTGTA
CAACCGAGCTCTGCCTGCCCTTCCCATATCCATCATGTTGGGTGTTGTTTTTTACAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCTAACG
ATTATTA
   
Sequence Name 43-K110925-0022-613-E06C-8409
GenBank Accession LN504873 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K110925-0022-613-E06C-8409
TTGTAAAAACAACACCCAACATGATGGATATGGGAAGGGCAGGCAGAGCTCGGTTGTACA
CAGATAACAAAATAAGAGTGCACCCAAGTCCGGAGATGATCGCAAGGTAACAGGCATACA
CAGTCATCAGATCATACATTGCTGCCCTACCAACAAGAACACTGTAGAAAATAAAGTCTC
CAAGACCAAGTTTTATGCCTCTATTTGAAATGTCCACAACGTTATCCGACTCCTCCAAAC
CTCTTGCTTCTTCCCTATTATCTCCCCAACCCATCAACTCAACTAGTGGAGACATTTCTT
CATCCATAACACTTTCTCTGTCTTCCATATTTCCTCTGG

Last Updated on 06.12.2018 16:11