Sequences from confirmation amplicon


Confirmation sequences for line 614B03 and hit At2g47610 are shown.
Line ID 614B03
Locus At2g47610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19530477
Primers used for PCR 08ih - 8474, show primer details
   
Sequence Name 64-K112758-0022-614-B03C-08ih
GenBank Accession LN511236 [GenBank]
Sequencing primer gene specific primer, 08ih: 5'-GTTGAGGCCGTATTTGACTACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K112758-0022-614-B03C-08ih
CTTGGGCCTTCTTTAAAGACGCTCCTTCTTGGCAGCTTTGTCTTCTGGCCTGTACTTCAG
AAGGACCTTGAAGAGGCTGGTAGCTGCAATTATCAATAACGAATATAAATAAGGCACCGA
AGTCTTCTTATAAGGTTACAGCAACACAAGATTTACCAGAACTGGTAAATATTTCAATAA
TAAATTAACAGCAGCAAGTAGTAAATGTTTGAAGAGTAGATTGAGAATGAAATAGACATA
CCAAGATTCTTGTCAAGAGTCTTGGTGAATTGGTTAAGAGCTGGAGGGACCTTCAACCTC
TGCTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC
   
Sequence Name 24-K112757-0022-614-B03C-8409
GenBank Accession LN497062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112757-0022-614-B03C-8409
ATTTACATTGAATATATCCTGCAGCAGAGGTTGAAGGTCCCTCCAGCTCTTAACCATTCA
CCAAGACTCTTGACAAGAATCTTGGTATGTCTATTTCATTCTCAATCTACTCTTCAAACA
TTTACTACTTGCTGCTGTTAATTTATTATTGAAATATTTACCAGTTCTGGTAAATCTTGT
GTTGCTGTAACCTTATAAGAAGACTTCGGTGCCTTATTTATATTCGTTATTGATAATTGC
AGCTACCAGCCTCTTCAAGGTCCTTCTGAAGTACAGGCCAGAAGACAAAGCTGCCAAGAA
GGAGCGTCTTGTAAAGAAGGCCCAAGCTGAAGCTGAGGGAAAGCCTTCTGAGTCTAAGAA
GCCCATTGTAGTCAAATACGGCCTCAA

Last Updated on 06.12.2018 16:11