Sequences from confirmation amplicon


Confirmation sequences for line 614F01 and hit At2g19780 are shown.
Line ID 614F01
Locus At2g19780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8523524
Primers used for PCR 03co - 8474, show primer details
   
Sequence Name 32-K108475-0022-614-F01C-03co
GenBank Accession LN500615 [GenBank]
Sequencing primer gene specific primer, 03co: 5'-AAGAATCATGAAGAAGAAAGGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K108475-0022-614-F01C-03co
CTCTGTCCTGAATTGTTTGATTCTAACAAACGGATACAGCATTGGAGTCGTAGTTGGAAT
CGGCGGTGGAGACGGTGACGACGGCGGAGTTTGGATAGGTGGAGGAAACCCTAATTCCAA
TCCTGGCTCAGCTCCAAACCAACAACAGACAACAAATGCAGCTTACAATGCTCTTCAATC
TTGGAAATCCGCCATTACAGAGGATCCATCTGGTGTTCTTAAAACATGGGTTGGTGAAGA
TGTTTGTTCTTACAGAGGCGTGTTCTGTTCTGGCTCATCTATAACCTCCATAGATCTAAA
CAAAGCAAATCTCAAAGGCACCATTGTTAAAGACCTCTCTTTACTCTCAGACCTAACCAT
TCTTCATCTCAACAGTAACAGATTCTCTGGTCAAATCCCAGATTCTTTCAAGAATCTTGA
TTCTCTTCAAGAACTCGATCTCAGCAACAACAGATTCTCCGGTTCTCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTA
   
Sequence Name 16-K108474-0022-614-F01C-8409
GenBank Accession LN493464 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K108474-0022-614-F01C-8409
TTTCATTGAATATATCCTGAGAACCGGAGAATCTGTTGTTGCTGAGATCGAGTTCTTGAA
GAGAATCAAGATTCTTGAAAGAATCTGGGATTTGACCAGAGAATCTGTTACTGTTGAGAT
GAAGAATGGTTAGGTCTGAGAGTAAAGAGAGGTCTTTAACAATGGTGCCTTTGAGATTTG
CTTTGTTTAGATCTATGGAGGTTATAGATGAGCCAGAACAGAACACGCCTCTGTAAGAAC
AAACATCTTCACCAACCCATGTTTTAAGAACACCAGATGGATCCTCTGTAATGGCGGATT
TCCAAGATTGAAGAGCATTGTAAGCTGCATTTGTTGTCTGTTGTTGGTTTGGAGCTGAGC
CAGGATTGGAATTAGGGTTTCCTCCACCTATCCAAACTCCGCCGTCGTCACCGTCTCCAC
CGCCGATTCCAACTCCGACTCCAATGCTGTATCCGTTTGTTAGAATCAAACAATTCAGGA
ACAGAGACAAAGACAGAAGGCTAAAGTTGTTGTTGTTGGTGCCTTTCTTCTTCATGATTC
T

Last Updated on 06.12.2018 16:11