Sequences from confirmation amplicon


Confirmation sequences for line 616A12 and hit At3g16480 are shown.
Line ID 616A12
Locus At3g16480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5602322
Primers used for PCR 34tg - 8474, show primer details
   
Sequence Name 36-K116871w-0022-616-A12C-34tg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 34tg: 5'-CTTCTTCACTCTTCAGCGAAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116871w-0022-616-A12C-34tg
CCCAAAATCAACACAAGATTTTAGAATAAGTAAAATGGATTTAAGTTTGCAATGCCGTTT
TATAAAAGATGTAAAACTGTGATGATGGTTTTGTCATTACCGTCTCCGAAAGTTGCCATT
GTCAAAGGCTTTGTGATAACCTTGCTAGTGAAATCTGCAATGTCCTTTAAGGTAAGTTGG
TCTACTGTCTTCAAGAATTGGTCTACTGGTTTCCTGCAGATATGTTGGGAAAACAAAAAA
AAAAACATTTAAAAAAAATTCGGGTTATAATCATTTTAAAAGTGAAATGTTACAAAGTGT
ATGTCCCTCTCTCCGAAATTAAAAATCTGTCTACCAATGTCTTCAATTGAAAAGGGCTTC
ATGTCCGGAAAATCTACTTGGATCACCATTGATTATAAGGGTCAATATGGAAAAAAAAAA
AAATTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATT
   
Sequence Name 16-K116870w-0022-616-A12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116870w-0022-616-A12C-8409
TAGACAGATTCTTACTTACGGAGAGAGGTACATACACTTTGTAACATTCCACTTCTAAAC
TGATTATAACCAGACTTTTCTCTAACTGTTTTTTTTTTTGTTTCCCCAACATATCTGCGG
GAAACCAGTAAACCATTTCTTGAAAACGGTAAACCAACTTCCCTTAAAGGACTTTGCAAA
TTTCCCTACCAGGGTTATCACAAACCCTTTGACAAGGGCAACTTTCGAAAACGGTAAGGA
CAAACCCTTCACCCCATTTTTACTTCTTTTATAAAACGGCTTTGCAAACTAAAACCCTTT
TAACTTATCCAAAAACCTGGGGTTAATTTGGGAACAGTGTTAAAGGTCCCAAGCTATAAC
CCCTTAACCAAAAAATTTCCCTA

Last Updated on 06.12.2018 16:11