Sequences from confirmation amplicon


Confirmation sequences for line 617F12 and hit At3g05130 are shown.
Line ID 617F12
Locus At3g05130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1434374
Primers used for PCR NG04 - 8474, show primer details
   
Sequence Name 24-K102364-0022-617-F12C-8409
GenBank Accession LN496940 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K102364-0022-617-F12C-8409
GCCTTTACATTGAAGGACAATCAAGGAATCTGAGGTTGAGAGGTTAATAGGAGAGAAGAA
TTTGATTGAGAAACAGATGGAGATGCTCAATGTACAGTCATCTGATAAGGGGAAGTTGAT
TGACCAGTTGTCTCGGGAGAAAGTTGAGCTTGAGGAGCGTATTTTTAGTCGGGAGAGGAA
GCTTGTTGAGCTGAATCGAAAGGCTGATGAGCTAACTCATGCTGTTGCTGTGCTGCAAAA
GAATTGTGATGATCAAACCAAGATTAACGGTAAGCTTAGCTGCAAAGTTGATCAGCTAAG
CAATGCTCTTGCGCAAGTTGAGCTCAGGAGAGAAGAAGCTGATAAGGCACTTGATGAGGA
GAAGAGAAATGGAGAGGATCTTAAGGCAGAGGTTTTGAAATCAGAGAAGATGGTTGCAAA
AACTCTAGAAGAGCTTGAGAAAGTTAAGATTGAGCGTAAGAGTTTATTTTCAGCGAAGAA
TGATTTGGAGAGTCGATCGAAATCATTG
Primers used for PCR 07ks - 8474, show primer details
   
Sequence Name 74-K118365-0022-617-F12C-8409
GenBank Accession LN513146 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118365-0022-617-F12C-8409
CATTACATTGAATATATCCTGATCAGTGTTCAAATCCCTCAAAACCTTCTCTTCCTTTGT
CAAACTCCGCACAGTCTCATTCAACTTGTCTAACTTTCTCTCCAGCTCCTCGATTACTCC
TTTTTGTTCAATCTTTACAATCTCCATCTCATTCTTCTCACTTAAAAGCACTTTGATCTC
TCTCTTCAATCCATCAATCTCATTCTTCTTCTCCTCCATTATCTTCTCCAGACCAATCTT
CTCCTTCTTTACCCCTTCAATCTCCTCTTCTCTCACCTTCCTCTCCTTCACCAATCTCTC
ATTTTC
   
Sequence Name 53-K118366-0022-617-F12C-07ks
GenBank Accession LN508297 [GenBank]
Sequencing primer gene specific primer, 07ks: 5'-GAAAATGAGAGATTGGTGAAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118366-0022-617-F12C-07ks
GAGGGGTAAGAAGAGAAATTGGTCTGGAGAAGATATGGAGGAGAAGAAGAATGAGATTGA
TGGATTGAAGAGAGAGATCAAAGTGCTTTTAAGTGAGAAGAATGAGATGGAGATTGTAAA
GATTGAACAAAAAGGAGTAATCGAGGAGCTGGAGAGAAAGTTAGACAAGTTGAATGAGAC
TGTGCGGAGTTTGACAAAGGAAGAGAAGGTTTTGAGGGATTTGAACACTGATCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAACGTTATTATAAT

Last Updated on 06.12.2018 16:11