Sequences from confirmation amplicon


Confirmation sequences for line 617H11 and hit At5g44840 are shown.
Line ID 617H11
Locus At5g44840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18105970
Primers used for PCR 19fx - 8474, show primer details
   
Sequence Name 21-K112248-0022-617-H11C-19fx
GenBank Accession LN495725 [GenBank]
Sequencing primer gene specific primer, 19fx: 5'-CCGCTGTTAATAGCTACACAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112248-0022-617-H11C-19fx
ATAATCTTAAATACCATTTGAGATTACTTTTTAATTAGTGAGTGTGCAAGAGAGATATCT
AGCTATATATACCAGTTCCAATAACTGTGTCGTATATATCGACATCACTTGATTCTGAGA
TATTAATTCCATCAGTGTTAGGACTATCGTGGGGTGCTACGAGTTTTATCTTCGTTATTT
TAACTCTTTTGCATTCGCTAATCGATATATGGTTTTTTGGACTGTTGAATGAGGTTATTT
CCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCC
   
Sequence Name 05-K112247-0022-617-H11C-8409
GenBank Accession LN488421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112247-0022-617-H11C-8409
GAGCATTTCATTGAATTATCCTGGAAATAACCTCATTCAACAGTCCAAAAAACCATATAT
CGATTAGCGAATGCAAAAGAGTTAAAATAACGAAGATAAAACTCGTAGCACCCCACGATA
GTCCTAACACTGATGGAATTAATATCTCAGAATCAAGTGATGTCGATATATACGACACAG
TTATTGGAACTGGTATATATAGCTAGATATCTCTCTTGCACACTCACTAATTAAAAAGTA
ATCTCAAATGGTATTTTAAGATTATTTAATTTTAAAATTTCTTATAAAACTTTCCAGGAG
ATGATTGTGTACTTTTAAACAGCGG

Last Updated on 06.12.2018 16:11