Sequences from confirmation amplicon


Confirmation sequences for line 620A03 and hit At2g27370 are shown.
Line ID 620A03
Locus At2g27370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11709349
Primers used for PCR CH79 - 8474, show primer details
   
Sequence Name 10-K107031-0022-620-A03C-8409
GenBank Accession LN490679 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K107031-0022-620-A03C-8409
TTACAATTGAATATATCCTGAATTTCGAGCAATGTGCTTGGTGCTTATTAGGGATTTAAT
TATGGTACATGATTTTCAATTATATAAAGATTCAAACTTATATCATTTTTTTTTATTGTT
TTGTTTTGCAGATCTTTTGTGATAGTAAACTCAATCGTGGGTGGCTACCTAACCCTCTCA
TTGCCTTTTTCTATAGTCTGTATCCTCCGCCCCCTCGCGGTGCCGCCTAGGCTATTCCTG
ATCTTATGTGATACGGTAACATTTATAAAAAAAATTTGAAAATAAATAGTTATAATAATG
CAATGCCAAACATACA
   
Sequence Name 26-K107032-0022-620-A03C-CH79
GenBank Accession LN497950 [GenBank]
Sequencing primer gene specific primer, CH79: 5'-TTGTATGTTTGGCATTGCATTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K107032-0022-620-A03C-CH79
TTTTTTTATAATGTACCGTATCACATAAGATCAGGAATAGCCTAGGCGGCACCGCGAGGG
GGCGGAGGATACAGACTATAGAAAAAGGCAATGAGAGGGTTAGGTAGCCACCCACGATTG
AGTTTACTATCACAAAAGATCTGCAAAACAAAACAATAAAAAAAAATGATATAAGTTTGA
ATCTTTATATAATTGAAAATCATGTACCATAATTAAATCCCTAATAAGCACCAAGCACAT
TGCTCGAAATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11