Sequences from confirmation amplicon


Confirmation sequences for line 620B11 and hit At5g21080 are shown.
Line ID 620B11
Locus At5g21080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7160789
Primers used for PCR 20wm - 8474, show primer details
   
Sequence Name 85-K118585-0022-620-B11C-20wm
GenBank Accession LN514591 [GenBank]
Sequencing primer gene specific primer, 20wm: 5'-GCAAGGCAATTGTATCTGTGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K118585-0022-620-B11C-20wm
TTCTTGTTGTTTTAGAATATATTTTCTGATTAGAGAGGGTAATAACAGCGGAGGATGCTA
AAAACCCCAAGTTTTGGTCGAGGGTTTGTCTGCATAATCTGGCAAAGTTAGCTAAAGAAG
CAACAACTGTGCGACGTGTTCTCGAGTCACTCTTCCGTTATTTTGATTTTAATGAAGTGT
GGTCCACCGAGAACGGTCTTGCTGTATATGTGCTGCAGGACGTTCAGTTGCTGATCGAAA
GATCTGGTTCGTATTGTCTCCGTTTGGTTTGTTTGTTTCTGGTCACCTTGTTAGTAACTT
ACTAATAGTGTGAGTACTTGACGACAGGGCAAAACACGCATTTCCTGTTGTCTATCCTGA
TCAAGCATCTTGATCACAAGAATGTTCTAAAGAAGCCTAGAATGCAACTTGAAATAGTTT
ATGTTGCTACTGCACTTGCCCAACAGACTAAAGTTCTGCCATCCGTGGCAATCATTGGTG
CCCTGAGTGATATGATTAGATGATTCAATTGGATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 55-K118584-0022-620-B11C-8409
GenBank Accession LN508944 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118584-0022-620-B11C-8409
ATTTACATTGAATATCCATTGAATCATCTAATCATATCACTCAGGGCACCTATGATTGCC
ACGGATGGCAGAACTTTAGTCTGTTGGGCAAGTGCAGTAGCAACATAAACTATTTCAAGT
TGCATTCTAGGCTTCTTTAGAACATTCTTGTGATCAAGATGCTTGATCAGGATAGACAAC
AGGAAATGCGTGTTTTGCCCTGTCGTCAAGTACTCACACTATTAGTAAGTTACTAACAAG
GTGACCAGAAACAAACAAACCAAACGGAGACAATACGAACCAGATCTTTCGATCAGCAAC
TGAACGTCCTGCAGCACATATACAGCAAGACCGTTCTCGGTGGACCACACTTCATTAAAA
TCAAAATAACGGAAGAGTGACTCGAGAACACGTCGCACAGTTGTTGCTTCTTTAGCTAAC
TTTGCCAGATTATGCAGACAAACCCTCGACCAAAACTTGGGGTTTTTAGCATCCTCCCTG
TTATTACCCTCTCTAATCAGAAAATATATTCTAAAAACAACAAGAAGCATGCCAAAGTAA
AGCTGGAGTAGAAGGATTTACACAGATACAATTGCCTTGC

Last Updated on 06.12.2018 16:11