Sequences from confirmation amplicon


Confirmation sequences for line 621C02 and hit At5g49850 are shown.
Line ID 621C02
Locus At5g49850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20260603
Primers used for PCR CH80 - 8474, show primer details
   
Sequence Name 11-K107031-0022-621-C02C-8409
GenBank Accession LN491135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K107031-0022-621-C02C-8409
TCGGACATGAGCCATTTACAATTGAATATATCCTGACATGCAGGAGGAAGAGGCATCGGA
TAAAAATGTGCACCAAGAGCCGTAATATACCCAGCCCCAAGAGGTGCATACCATCCATGA
AATCCGACAACGGCACAACCTTTGCTCTCAATGACAAATTCAACTGAATCAGAAGTTCTC
TCACCAAATGTCCTAGAGGTTCTCCCTTTTGATGTTTTGAAAACAAATGACGTAACATAA
CTATCATTCATGGTCCCCACAATGGACGTGATAACTTCATATGGATAGTCAACAACAAAC
TGCAATAACCAGAATAATTAAGCTATTCAACAACACCTACATATATGTATATTTTAGTTG
AGGGACTTATAGATATAAGTATTCTCACCTCGTCAGTGATTCGCGAATCGTCGTTCATTC
CATGGGCACGCTTTACTACTTTGCCATCGTTTTCATAGTTGATCCGGAAACACCGTATAC
AATAAGATCTCCATGTAACGGAAACCTTTCTAATACCTTCAAAAGCACCACTATCCCAAA
GGGTGCTTCGTTCAGTAACTCCTTGGCATTCTGACTTTGTTGGAATAATCGGGNTGAAAT
   
Sequence Name 27-K107032-0022-621-C02C-CH80
GenBank Accession LN498409 [GenBank]
Sequencing primer gene specific primer, CH80: 5'-TATTTCACACCGATTATTCCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K107032-0022-621-C02C-CH80
TGACGAAGCCCCTTTGGGATGTGGTGCTTTTGAAGGTATTAGAAAGGTTTCCGTTACATG
GAGATCTTATTGTATACGGTGTTTCCGGATCAACTATGAAAACGATGGCAAAGTAGTAAA
GCGTGCCCATGGAATGAACGACGATTCGCGAATCACTGACGAGGTGAGAATACTTATATC
TATAAGTCCCTCAACTAAAATATACATATATGTAGGTGTTGTTGAATAGCTTAATTATTC
TGGTTATTGCAGTTTGTTGTTGACTATCCATATGAAGTTATCACGTCCATTGTGGGGACC
ATGAATGATAGTTATGTTACGTCATTTGTTTTCAAAACATCAAAAGGGAGAACCTCTAGG
ACATTTGGTGAGAGAACTTCTGATTCAGTTGAATTTGTCATTGAGAGCAAAGGTTGTGCC
GTTGTCGGATTTCATGGATGGTATGCACCTCTTGGGGCTGGGTATATTACGGCTCTTGGT
GCACATTTTTATCCGATGCCTCTTCCTCCTGCATGTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11