Sequences from confirmation amplicon


Confirmation sequences for line 621F10 and hit At5g38780 are shown.
Line ID 621F10
Locus At5g38780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15530229
Primers used for PCR 61rn - 8474, show primer details
   
Sequence Name 36-K116132-0022-621-F10C-61rn
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 61rn: 5'-TGGCTTTCTTTGAGGTGTTTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116132-0022-621-F10C-61rn
ATCCCTAGCCCCATACGAATGATGTCAGGATATGTTCAATAGTAAGTGGCTTCATGTCCA
GTAAATCCACATGGATCAGCAATGAGTATGATGGTCTCCTATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 11-K116131-0022-621-F10C-8409
GenBank Accession LN491272 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116131-0022-621-F10C-8409
AACCATAACATGCAATTTTTTGTGAGAACTAATCCCTAAATATATCTAGTACACTGTAAT
GATTAACATACCTTTGTGATACAGAAAGCAGGCATAGATGGGGTTCAAGAAAAGGCAAGA
CAATACATTTTAGAAAACCTCGATCTCCTTAATATGAATCCCAATCTTAGTACTTTTACA
ATTGCAGATTTTGGTTGTTCTATTGGCCCTAACACTTTTCATGCTGTTCAAAATATCATT
GATATTGTGAAACTTAAACACCTCAAAGGAAAGCCA

Last Updated on 06.12.2018 16:11