Sequences from confirmation amplicon


Confirmation sequences for line 622B07 and hit At2g39960 are shown.
Line ID 622B07
Locus At2g39960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16682567
Primers used for PCR 29vh - 8474, show primer details
   
Sequence Name 78-K117751-0022-622-B07C-29vh
GenBank Accession LN513740 [GenBank]
Sequencing primer gene specific primer, 29vh: 5'-GAAGCAATCCCACTAACCAAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K117751-0022-622-B07C-29vh
TTATGTTTCCTAGAGCTAACTAAACGAGTACAGAATGAAATGTTTGGAAAAAAAAATCTC
ATCGTCTCGGATACTAACTAAATGAGAGGAGTAATTTCTTCAAGCTGGTAAGAATGAGAA
ATAAACCACCCAAGTTACAGTCCAGAATCCTCCACTTAGTTGAACAATTATGTTTCGAGA
AACAAGACAGAAACGATTGCAAACATCAAAAACTTTTGTTCAACTATTTTTCAAAATCTA
AAGTTTGAAGACTAAAACTAATTGAACAATACATTGAAAGGGAAATGTAGTTTTGCCTAG
AGCTCAAATGAAAAAAAAAAAAAAAAAAAAAGGGCCTTAAAAATTGGAAAGTTGGAAATT
TTTAAAAAAAGGGGGGGGGGGCCAAAAAAAAATTCAAAAAAAAGGGGAGGGAAAATTTTG
ACCCCCAAATTTAAACTTTTCCCCCCTTTAAAAATAAGGGAAAAGGGAAAAAGGGGTCTT
TTTGCCCCTTATTCCCAAAAAAACAAAACCTTTTGAAAGGGGTTCTTGTCCGGGAAATTC
CCCGGGGACCCCCAGGGGTTAGGGGGGCAATTGGGGAAAAAAAAAAAAATTTTTCCCCTT
TTTTTTTCCATTCAAAAATGGA
   
Sequence Name 53-K117750-0022-622-B07C-8409
GenBank Accession LN508284 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117750-0022-622-B07C-8409
TTTTCTGTTGATTTAGGTTGCATAATGTACCTTTTTTCCTTGTTCACTTACTTTTAACTG
GAGCTATAGTTTGAATTCTGGTGTCAAACTTTTGCATTCCTTTTGTTTTGTATTTTGTTT
CTGCTTGCCTGACATGTTATGTAAAGTTTGCAACTTTGCATATCTTAAGGCATCTTTTTT
TTTTTTTTTTTTTTCCTTTGACCCCCGGGAAAAAAAAATTTTCCCTTTTAATTTTTTTTT
CAATTTTTTTTTTTTTTCAAATTTTAATTTTTAAAAAAATTTTAAAAAAAATTTTTTTAT
TTTTGCAACCTTTTTTTTTTTTTTTCCCAAAAAAAATTTTTTCAACAAAGGGGAAAATTT
CGGACTGAAACTGGGGGGGTTTTTTTCCCCTTTCCCCCCCCTTAAAAAAATTCCCCCCCC
CCTTTTTTTTATACCCAAAAAAAAAAATTTTTTTTTTCCAAAATTTTTATTTTGAACCCT
TTTAATTCCCCTTGGAAAAAAAAAAAAAAAAACCCCAAAATTTTTTTTTTTTTAAACCCC
TTTTAATTTTGGCCAAAATTTTT

Last Updated on 06.12.2018 16:11