Sequences from confirmation amplicon


Confirmation sequences for line 623B03 and hit At3g45275 are shown.
Line ID 623B03
Locus At3g45275 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16606198
Primers used for PCR 24qp - 8474, show primer details
   
Sequence Name 67-K115691-0022-623-B03C-24qp
GenBank Accession LN511880 [GenBank]
Sequencing primer gene specific primer, 24qp: 5'-ACATGTATGCACAAAATCCAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K115691-0022-623-B03C-24qp
AGGGGTCAACATTATACACTATTATAAATACTATGAAAGAATGTTACGATTACCATCACA
AGACAGACTAGAAATTCTATAAACACTCGAAATGAGCGGGAACAAGCGAGCGTCTGTGTC
CATCTTAGTAGTGATGATAATGATGCTATCCATTTCCCAAATTACGAAAGGTAATAATGA
TTTTGCCTTTGCTCCAATGTCAAAAAAAGGATTGTTGCCAAAACCATTGTCTTGTGCGAC
AGACGCAGGAAAAATTCCGGATTGTGTAGAAGCAGTGAAGCATTTCAAATTTAAAAATGT
CAAAAAAGAATGTTGCTTTATACTACTCGGTCTTACCGAAGACTGTTTTGGAATTTTGTT
TCCTATGCGTTTTGCGTATCGTGAAATGCTTAAGATTACATGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 19-K115690-0022-623-B03C-8409
GenBank Accession LN494898 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115690-0022-623-B03C-8409
ATCTTAAGCATTTCACGATACGCAAAACGCATAGGAAACAAAATTCCAAAACAGTCTTCG
GTAAGACCGAGTAGTATAAAGCAACATTCTTTTTTGACATTTTTAAATTTGAAATGCTTC
ACTGCTTCTACACAATCCGGAATTTTTCCTGCGTCTGTCGCACAAGACAATGGTTTTGGC
AACAATCCTTTTTTTGACATTGGAGCAAAGGCAAAATCATTATTACCTTTCGTAATTTGG
GAAATGGATAGCATCATTATCATCACTACTAAGATGGACACAGACACTCGCTTGTTCCCA
CTCATTTCAAGTGTTTATAGAATTTCTAGTCTGTCTTGTGATGGTAATCGTAACATCTTT
CATAGTATTTATAATAGTGTATAATTATGACCCTTTTAGTAGACGATCTATATTGTTTTT
TTCTTGGATTTT

Last Updated on 06.12.2018 16:11