Sequences from confirmation amplicon


Confirmation sequences for line 623B08 and hit At2g21060 are shown.
Line ID 623B08
Locus At2g21060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9037498
Primers used for PCR DC21 - 8474, show primer details
   
Sequence Name 19-K031248-022-623-B08C-8409
GenBank Accession LN494643 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K031248-022-623-B08C-8409
GCCTTTACAATTGAATATATTGATGATCAATGTACAATTGAAACCAAACCCCTTCTGTGT
ATCAAACCACTTCACCGTTCCCTTGCGTCTGTCTCCACCACTCATGTTCACGTCTCCTCC
TCCGCTCATTCTGATCAAAATCTCTCCGACGAACTAAAAGTAAATCACCGATCAGATCTC
TCTCTCTCTCCTTTGGCGTGAAGCAGACGCTGTTTATATATCTGCGAGATATGTGTGTAA
AGGCATAGGGTATCCCGCCACGTCACTATTTACTTTTTATTCCAATATTCTCTACTCTTT
TTTCTTTCATATTCCCATTTTTAATACGTATTTTCCATCTTATAAATTGGCTATTTCTAT
TTTTCTGCATAATTCCTTGACTTTTTCCAGTACAATTAGAAATTTTACTTAATGAAATAC
GAATTTGTTTGAATAGTAAAACTAAAAAGGAAATAATATAATTACAAAAATGGGCCAGGC
TAATGTTCGAGATTGTATTATTTTTTCCTTTTCGGGAAACTAAAGGGAGCGATGGGCCCG
CTTTGATCTGGACGGTTGGGGATGCTTTGCAGGAGGCTTCTCAGCCGTAGNTTTCAAAT
   
Sequence Name 20-K031248-022-623-B08C-DC21
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, DC21: 5'-GATTTGAATCTAACGGCTGAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K031248-022-623-B08C-DC21
ATTGAGGTGCTTTTTTTTGTAAGAGGGAGGGAAAATGGAGAATTCCGTA

Last Updated on 06.12.2018 16:11