Genotyping and primer details


Genotyping and primer details for 623B08 - At2g21060
Line ID 623B08
Locus At2g21060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9037498 of line 623B08 Chr2:9037498
   
Insertion allele amplicon 8474 - DC21
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DC21: 5'-GATTTGAATCTAACGGCTGAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9038059
   
wt amplicon (over InsPos) 16ho - DC21
wt amplicon start Chr2:9036945
wt amplicon end Chr2:9038059
wt amplicon size 1115 bp
Locus specific primer DC21: 5'-GATTTGAATCTAACGGCTGAGAAG-3'
2nd locus specific primer 16ho: 5'-GATGACTGATTTACCCCTTCTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11