Sequences from confirmation amplicon


Confirmation sequences for line 623C07 and hit At3g24068 are shown.
Line ID 623C07
Locus At3g24068 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8692366
Primers used for PCR 82we - 8474, show primer details
   
Sequence Name 79-K118544-0022-623-C07C-82we
GenBank Accession LN513878 [GenBank]
Sequencing primer gene specific primer, 82we: 5'-AGGTGATGTTGTTGAAGTGTTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K118544-0022-623-C07C-82we
GATGATGAAACGCAATGGTTACCACAATTATTTCAGATTTTAAGAATGAGATAATTTCAT
AATTAAAAAAAAAAAAAAAATTTTTTGATGATGTTAAGTATCGGTTCTTAAAGGGATAAC
ATCGAGTTAGCCAACTTTCGTATTTGTGATATGAAAAAAAAAAAAAATTGAATCAACCGT
ATGTTTTCGTTACAACCCCTAACCCAAGTTCTTTTCGGGGATTTCTTCTTAGGAACTAAA
TCATATCAAAATTTTAAAAATCCCTTAAAAATCTTATAATTATTTTATTTTATCCGACGG
AACGATACAATAACCCAAGGCAAATGGTTGGGGGTATCACCAATTGAATTGCTTTTGGTA
TTTCTGAATAAAAGCGACTTATGGGTAAAGACCATCGGGATTGATTTGCCATAAACAAAA
ACCATCGAATCTGCATCAGGATATTTTCAATTGTAAAGGGCTTCATGTCCGGGAAATCTA
CTGGGATCACCAATGAGTATGAGGGTCAATATGGAGAAAAAAAAAGAGTATTTCCCATTT
TTTTTTCATTTCAAAAATGTAAATTT
   
Sequence Name 59-K118543-0022-623-C07C-8409
GenBank Accession LN510108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118543-0022-623-C07C-8409
TGATGCAGAATCCGAAGGTTTTTGTCTATGGCAAATCAATCCTGATGGTCTTTACCTATA
TGTCGCTTCTATTCAGAAATACCAAAAGCAATTCAATTGGTGATACACCAAACAATATGC
ATTGTGTTATTGTATCGTTCAGTCGTATAATATAATATAATTATAAGATTTCTAAGGTAT
TTTTAAATTTATGATATGATTTAGTTCATAAGAAGAAATCACAGAAAAGAACTTGGTTTA
GGTGTCGTAACGAAAACATACGTATGATTCAATTTTTTTTCTTCTTCATATCACATATAC
GAAAGTTGGCTAACTCGATGTTATCACTTTAAGAACCGATACTTAACATCATCATAAATT
TTTTTTTTTTTTTTTAATTAGAAAATTATCCCATTCTTAAAATCTAAAATAATTGGGGAA
ACCATTGCGTTTCACCATCCTTTCGTCTTTTTTGAAAAAGTTTCGTCATCAAACAGTTTC
CAACGTAACAT

Last Updated on 06.12.2018 16:11