Sequences from confirmation amplicon


Confirmation sequences for line 623G01 and hit At3g59700 are shown.
Line ID 623G01
Locus At3g59700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22053533
Primers used for PCR JA09 - 8474, show primer details
   
Sequence Name 89-K100942-0022-623-G01C-8409
GenBank Accession LN514928 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K100942-0022-623-G01C-8409
CTTTACATTGAATTATCCTGATAGTCGTCAATTCCGTCAATATATGTTGAGAATGATGAC
TTGTACCCATTCTTGATGCAGGTGTAGTAGAGCAGTTGCAACATCTTTGATGATCCTGAA
ACGTTGTTCCCAAGTAAGCCGTTCTTGATTCTCGCTACGGTTTAGATACTTGTCAAGGCT
TCCATTAGGCATATAGTCATACACCAAGTAGAGATTCTCTTTATGCCTACAGTATCCTAA
AAGCCTGACTAAATTTGGATGTCTGAGACGACCAATGGTCGATATCTCGGCTAGAAACTC
GCTCATCCCTTGTCTTGAATCATGAGAAGTCCGCTTCACAGCGATCTCTGCATCAGAACC
CGGAAGTGTTCCTTTATAGACTTGACCAAAGCCTCCTTTACCAAGAAGTTGTTTCTCCTT
GAAACCCTTTGTGGCATTGAAAAGCTCCTTATAAGCAAACCTATGAGGTCCATATTGAAT
CTCCCATTCTTCAAGAACCTCTNTAACCTTCTTATGCCTCANATAGAAGACGAAACCGAT
CCACGAAGCGACNAACGCAGCANACACAGATACCGTTNAGCAGACGGCTAANACAGTTTT
CGTTCTATTAGATGTTTTCTTT
   
Sequence Name 89-K100950-0022-623-G01C-JA09
GenBank Accession LN514929 [GenBank]
Sequencing primer gene specific primer, JA09: 5'-TCCTCCATATCCAAAGAAAACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K100950-0022-623-G01C-JA09
CTTACGGTATCTGTGTTTGCTGCGTTTGTCGCTTCGTGGATCGGTTTCGTCTTCTATTTG
AGGCATAAGAAGGTTAAAGAGGTTCTTGAAGAATGGGAGATTCAATATGGACCTCATAGG
TTTGCTTATAAGGAGCTTTTCAATGCCACAAAGGGTTTCAAGGAGAAACAACTTCTTGGT
AAAGGAGGCTTTGGTCAAGTCTATAAAGGAACACTTCCGGGTTCTGATGCAGAGATCGCT
GTGAAGCGGACTTCTCATGATTCAAGACAAGGGATGAGCGAGTTTCTAGCCGAGATATCG
ACCATTGGTCGTCTCAGACATCCAAATTTAGTCAGGCTTTTAGGATACTGTAGGCATAAA
GAGAATCTCTACTTGGTGTATGACTATATGCCTAATGGAAGCCTTGACAAGTATCTAAAC
CGTAGCGAGAATCAAGAACGGCTTACTTGGGAACAACGTTTCAGGATCATCAAAGATGTT
GCAACTGCTCTACTACACCTGCATCAAGAATGGGTACAAGTCATCATTCTCAACATATAT
GGACGGAATTGACGACTATNANGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTA
Primers used for PCR 20xf - 3144, show primer details
   
Sequence Name 20-K118953-0022-623-G01C-20xf
GenBank Accession LN495438 [GenBank]
Sequencing primer gene specific primer, 20xf: 5'-CAGTAGATTGGACTTTTGACAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118953-0022-623-G01C-20xf
AGATTAATATCTATATCACATCTGTTTAGGGTTCTTTGAATTCATGAACCATAGAAACAT
AATCGGAGCTTTGTTTGATCACGTTCTTCTATTTGGATAATTTCTGCAGATACTCCTAAC
GAATTGGCGTATCAAAGAGGAAACAGTATAGTATTATAAGATTTCTTAACCATTCAATTT
CATAGGATTTACCGGACAATGGTCTCTAAGAAAAAGAATTGATTTTCAAGATTAATAATC
GAGTTGTTATCAAATACAAAAGGATTAAAAAAATGTTTGGAGGAGGCGGTGTGGTCTTAT
TCTTGTACATACCAGGAAAAAAAACATTCGGCTGTGTATTACAATGTGTAATAATCTCAG
TTGTGATACATCTTCATAGCAAACAAAGAATCAGTGGTAAACTTAAAATTTCAGCGGCCG
TGGGAGACAAAAGAAGAGTCGGTTAACTCCAATGAACTCGATGTATTCACATCAAGTAGT
AGTAATTCCATTGATGTCTCAGGCCATTCTCTAAATTTCTCAGCCCTTACAACATCAAGA
AGATTATCTGGTAGCTGTGAAACTCCATTCAAAATCCTCATTACCACACTCATAGCCGGT
CTTATCGATGCAGCTTGATGCGAACACAACACACCTAGCTTCAAAACAAGCTCAACTTGT
CCCCTGTTTTGTTCTTGACGTATACTTTCCTCTGCTGCATCGAANATTTTCCCATTTTCC
CAAAGCTCTAAGATCCAATCCACAAGATATTCTTCATTTTCTGCTGCACGTCTCTCAATT
ATCCTTCTACCACACACTACTTCAAGCATTTACCACCCCAAGGCGTAAACATCAGTGCTT
GTGGTTGCTCTTCTTGTTCTTAGAAACTCTGGTGCGATATATCCGAATGTTCCCGCTACT
TTAGATGTTTCAGGA
   
Sequence Name 04-K118952-0022-623-G01C-3144
GenBank Accession LN488129 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118952-0022-623-G01C-3144
TCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGATATCAAA
CCAGCTAATGTTTTAATCGACAATGAAATGAATGCGAGGCTCGGGGATTTCGGACTGGCG
AAACTGTATGATCAGGGATTCGATCCTGAAACATCTAAAGTAGCGGGAACATTCGGATAT
ATCGCACCAGAGTTTCTAAGAACAGGAAGAGCAACCACAAGCACTGATGTTTACGCCTTT
GGGTTGGTAATGCTTGAAGTAGTGTGTGGTAGAAGGATAATTGAGAGACGTGCAGCAGAA
AATGAAGAATATCTTGTGGATTGGATCTTAGAGCTTTGGGAAAATGGGAAAATTTTCGAT
GCAGCAGAGGAAAGTATACGTCAAGAACAAAACAGGGGACAAGTTGAGCTTGTTTTGAAG
CTAGGTGTGTTGTGTTCGCATCAAGCTGCATCGATAAGACCGGCTATGAGTGTGGTAATG
AGGATTTTGAATGGAGTTTCACAGCTACCAGATAATCTTCTTGATGTTGTAAGGGCTGAG
AAATTTAGAGAATGGCCTGAGACATCAATGGAATTACTACTACTTGATGTGAATACATCG
AGTTCATTGGAGTTAACCGACTCTTCTTTTGTCTCCCACGGCCGCTGAAATTTTAAGTTT
ACCACTGATTCTTTGTTTGCTATGAAGATGTATCACAACTGAGATTATTACACATTGTAA
TACACAGCCGAATGTTTTTTTTCCTGGTATGTACAAGAATAAGACCACACCGCCTCCTCC
AAACATTTTTTTATCCTTTTGTAATTTATAACAACT
Primers used for PCR 70xe - 3144, show primer details
   
Sequence Name 19-K118953-0022-623-G01C-70xe
GenBank Accession LN494981 [GenBank]
Sequencing primer gene specific primer, 70xe: 5'-TCAATTTCATAGGATTTACCGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118953-0022-623-G01C-70xe
TCAGATAATAATCGAGTTGTTATCAAATACAAAAGGATTAAAAAAATGTTTGGAGGAGGC
GGTGTGGTCTTATTCTTGTACATACCAGGAAAAAAAACATTCGGCTGTGTATTACAATGT
GTAATAATCTCAGTTGTGATACATCTTCATAGCAAACAAAGAATCAGTGGTAAACTTAAA
ATTTCAGCGGCCGTGGGAGACAAAAGAAGAGTCGGTTAACTCCAATGAACTCGATGTATT
CACATCAAGTAGTAGTAATTCCATTGATGTCTCAGGCCATTCTCTAAATTTCTCAGCCCT
TACAACATCAAGAAGATTATCTGGTAGCTGTGAAACTCCATTCAAAATCCTCATTACCAC
ACTCATAGCCGGTCTTATCGATGCAGCTTGATGCGAACACAACACACCTAGCTTCAAAAC
AAGCTCAACTTGTCCCCTGTTTTGTTCTTGACGTATACTTTCCTCTGCTGCATCGAAAAT
TTTCCCATTTTCCCAAAGCTCTAAGATCCAATCCACAAGATATTCTTCATTTTCTGCTGC
ACGTCTCTCAATTATCCTTCTACCACACACTACTTCAAGCATTACCAACCCAAAGGCGTA
AACATCAGTGCTTGTGGTTGCTCTTCCTGTTCTTAGAAACTCTGGTGCGATATATCCGAA
TGTTCCCGCTACTTTAGATGTTTCAGGATCGAATCCCTGATCATACAGTTTCGCCAGTCC
GAAATCCCCGAGCCTCGCATTCATTTCATTGTCGATTAAAACATTAGCTGGTTTGATATC
TCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCAAATGAAATGAACTTCTTATATAGAGGAAGGGTCTTGCG
   
Sequence Name 03-K118952-0022-623-G01C-35St
GenBank Accession LN487699 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118952-0022-623-G01C-35St
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGATATCAAACCAGCTA
ATGTTTTAATCGACAATGAAATGAATGCGAGGCTCGGGGATTTCGGACTGGCGAAACTGT
ATGATCAGGGATTCGATCCTGAAACATCTAAAGTAGCGGGAACATTCGGATATATCGCAC
CAGAGTTTCTAAGAACAGGAAGAGCAACCACAAGCACTGATGTTTACGCCTTTGGGTTGG
TAATGCTTGAAGTAGTGTGTGGTAGAAGGATAATTGAGAGACGTGCAGCAGAAAATGAAG
AATATCTTGTGGATTGGATCTTAGAGCTTTGGGAAAATGGGAAAATTTTCGATGCAGCAG
AGGAAAGTATACGTCAAGAACAAAACAGGGGACAAGTTGAGCTTGTTTTGAAGCTAGGTG
TGTTGTGTTCGCATCAAGCTGCATCGATAAGACCGGCTATGAGTGTGGTAATGAGGATTT
TGAATGGAGTTTCACAGCTACCAGATAATCTTCTTGATGTTGTAAGGGCTGAGAAATTTA
GAGAATGGCCTGAGACATCAATGGAATTACTACTACTTGATGTGAATACATCGAGTTCAT
TGGAGTTAACCGACTCTTCTTTTGTCTCCCACGGCCGCTGAAATTTTAAGTTTACCACTG
ATTCTNTGTTTGCTATGAAGATGTATCACAACTGAGATTATTACACATTGTAATACACAG
CCGAATGTTTTTTTTCCTGGTATGTACAAG

Last Updated on 06.12.2018 16:11