Sequences from confirmation amplicon


Confirmation sequences for line 623H02 and hit At1g12100 are shown.
Line ID 623H02
Locus At1g12100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4095295
Primers used for PCR 54mk - 8474, show primer details
   
Sequence Name 48-K114277-0022-623-H02C-54mk
GenBank Accession LN506572 [GenBank]
Sequencing primer gene specific primer, 54mk: 5'-AACATGCTTGATGTGATGAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114277-0022-623-H02C-54mk
CGGAATAGGCCCTGTCTAAGTTAAAATTTTAACAATCGAACAATCAAACCCGGAAAAAGA
TTAAGGATCTTAGTTGAACCAGTTGAGTCATACACACTGATTACGAATCACAAATCAAAC
TGTAGTATAAAACAATTGCAACTTGGCTTTCCACCCCCACACATGATTTTTGTTATTGTT
CATAAGAAAACAAAGTATTTCTTATTATTATTCTTCTTTAAAAACAATTCTTATTATTAT
TCACTTCACGGGATTACATAAGAATTCTAAATCGCCCATTGGTTCATCTTGTGATCCATT
GGATGGCAAATGATATTAAATTATGTTTTATTCGGGTAATGGATCTATAAAATTATCGGT
TTATTTTTATATAACAAAAAAGGTAACGAAAAATATTGTTTATCATCAAGCGGTCTTTGC
CTAATTAATGACGTTTTACAAAAAAAACTCTGAAGGTTGTAATTCATATGATCCCTAACT
GGAAAATATGCGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCC
   
Sequence Name 18-K114276-0022-623-H02C-8409
GenBank Accession LN494401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114276-0022-623-H02C-8409
TCGCATATTTTCCAGTTAGGGATCATATGAATACAACCTTCAGAGTTTTTTTTGTAAAAC
GTCATTAATTAGGCAAAGACCGCTTGATGATAAACAATATTTTTCGTTACCTTTTTTGTT
ATATAAAAATAAACCGATAATTTTATAGATCCATTACCCGAATAAAACATAATTTAATAT
CATTTGCCATCCAATGGATCACAAGATGAACCAATGGGCGATTTAGAATTCTTATGTAAT
CCCGTGAAGTGAATAATAATAAGAATTGTTTTTAAAGAAGAATAATAATAAGAAATACTT
TGTTTTCTTATGAACAATAACAAAAATCATGTGTGGGGGTGGAAAGCCAAGTTGCAATTG
TTTTATACTACAGTTTGATTTGTGATTCGTAATCAGTGTGTATGACTCAACTGGTTCAAC
TAAGATCCTTAATCTTTTTCCGGGTTTGATTGTTCGATTGTTAAAATTTTAACTTAACAG
GGCCTATTCCGCCTTATTCGGCTCCTAGTTGTTCTTTTAAAATCTTATTATGCTTCTTCA
T

Last Updated on 06.12.2018 16:11