Sequences from confirmation amplicon


Confirmation sequences for line 624F12 and hit At1g78850 are shown.
Line ID 624F12
Locus At1g78850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29643364
Primers used for PCR CH86 - 8474, show primer details
   
Sequence Name 50-K107248-0022-624-F12C-CH86
GenBank Accession LN507185 [GenBank]
Sequencing primer gene specific primer, CH86: 5'-ATTATTTCGTTCGATTCAATACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K107248-0022-624-F12C-CH86
TTACTATGCTGATGCAGTGTAAGAAGTATAGTTATCAACCAACAACTCGCTTTGTAAGAG
AAACATATCATACTTTTGATGATACTATTTTTTCTTTTTTGAATTTCACACCGGATTCAA
TCGGTAAAGTATTATAGAAGTTCTTACTGAAACAACATTAATTTTTTTGTAAAATATAAA
TCTAAGAAGAAAAAATTACTAGGGAAATTAATAGCAAACTGTAACGGTTGACTGGTTTTG
CTAATCGTGCACGGTTTAGCATTGAGATTAAGATAAGCTCTTTCTAAAATATTCGAAACA
TATCCTTTAAAAAAAAAAAAAAAAATCAAACAAACCCCGCAAAAGTAATAAATTTTGGCA
AAAAAAAAACCAAAAAGTCGTTCAAAATACTTTTTAAAATTTTAAAAAACCAACAAAAAA
ACAAAAACTTCCTGGGCCCCCCCAAAAAAAAAAAGTTTTTTCTTTTTCCCAAAAACCCCC
CAAAATCCTTTAACCCCCCCAAAAACAAAAGGAATTTTTCAACCCCTTTAGCTCTTTGCT
TAAATTGAAAAGGGCTTCATGTCCGGGAAATCTCCTGGGATCACCAAGGAGTTTGAGGGT
CAATTGGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAAGGTAAA
   
Sequence Name 34-K107247-0022-624-F12C-8409
GenBank Accession LN501439 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K107247-0022-624-F12C-8409
TTACAATTGAAGCATAGAGCTAATGTGATTGAAAATTTCATTTTGTTTTTGTGTGTGTTA
ATGGATTTTGGTGTGTTTCTGGTAAAGAGAGAAAGCTTCTATTTATAGGAGGAGCCAAGG
AAGTCTTTGTTTTTTTGTTGGTTTTATAATATTTTAAAGAGTATTTTGAACGACTTTTTG
GTCTTTGTTTCGCTATAATCTATTACTTTTGCTGGTTTTGTTTGATTTTTTTTTTTTTTT
TTAAAGGATTTTTTTCAAATTTTTTAAAAAAAGTTTTTTTTATTCTCAATGCTAAACCGG
GCACAATTAGAAAAACCAGTCAACCGTTACATTTTGCTTTTAATTCCCCAAGAAATTTTT
TCTTCTTAAATTTATTTTTTACAAAAAAATTAAGGTTGTTTCAGAAAAAACTTCTAAAAT
ACTTTCCCGATTGAACCCGGGGGGAATTTCAAAAAAAAAAAAATATTTTCCCCAAAAGTT
GGATTTGTTTCTTTTACAAAGCGAGTTGTGGGTTGAAAACTATACTTTCTTACCCGGCAT
CACCTTTATTAAATTTGAAATTCCTAAATTTCCGTTTTGAACCAAAAAAAATAAAA

Last Updated on 06.12.2018 16:11